Spry2 Rabbit Polyclonal Antibody
Catalog# HA500170
Spry2 Rabbit Polyclonal Antibody
-
WB
-
IHC-P
-
Human
-
Rat
-
unconjugated
Overview
Product Name
Spry2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human Spry2 aa 1-180.
Species Reactivity
Human, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 35 kDa
Positive Control
Rat liver tissue lysates, human liver tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500
-
IHC-P
-
1:200
Target
Function
This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein.
Background References
1. Zhu Y. et. al. ASIC1a regulates miR-350/SPRY2 by N(6) -methyladenosine to promote liver fibrosis. FASEB J. 2020 Nov
2. Yawut N. et. al. Overexpression of Cancer Upregulated Gene 2 (CUG2) Decreases Spry2 Through c-Cbl, Leading to Activation of EGFR and beta-Catenin Signaling. Cancer Manag Res. 2020 Oct
Subcellular Location
Ruffle membrane, cytoskeleton.
Synonyms
hSPRY 2 antibody
hSPRY2 antibody
MGC23039 antibody
Protein sprouty homolog 2 antibody
Sprouty 2 antibody
Sprouty homolog 2 (Drosophila) antibody
Sprouty homolog 2 antibody
Sprouty2 antibody
Spry 2 antibody
Spry-2 antibody
ExpandImages
-
Western blot analysis of Spry2 on rat liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500170, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Predicted band size: 35 kDa
Observed band size: 35 kDa -
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Spry2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500170, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"