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SF3A1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500175

SF3A1 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

SF3A1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human SF3A1 aa 744-793.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 89 kDa

Positive Control

HL-60 cell lysate, Daudi cell lysate, Hela cell lysate, 293T cell lysate, mouse ovary tissue, Hela, mouse brian tissue, mouse lung tissue.

Conjugation

unconjugated

RRID

AB_3071272

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500

  • IHC-P

  • 1:500-1:1,000

Target

Function

Involved in pre-mRNA splicing as a component of the splicing factor SF3A complex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes. This gene encodes a subunit of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP). U2 small nuclear ribonucleoproteins play a critical role in spliceosome assembly and pre-mRNA splicing.

Background References

1. Martelly W. et. al. Identification of a noncanonical RNA binding domain in the U2 snRNP protein SF3A1. RNA. 2019 Nov

2. Tian J. et. al. SF3A1 and pancreatic cancer: new evidence for the association of the spliceosome and cancer. Oncotarget. 2015 Nov

Subcellular Location

Nucleus, Nucleus speckle.

UNIPROT

SwissProt: Q15459 Human

SwissProt: Q8K4Z5 Mouse

Synonyms

Pre mRNA processing 21 antibody

Pre mRNA splicing factor SF3a antibody

PRP21 antibody

PRPF21 antibody

SAP 114 antibody

SAP114 antibody

sf3a1 antibody

SF3A1_HUMAN antibody

SF3a120 antibody

Spliceosome associated protein 114 antibody

Expand

Images

  • Western blot analysis of SF3A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500175" style="font-weight: bold;text-decoration: underline;">HA500175</a>, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><br />Positive control: <br />Lane 1: HL-60 cell lysate<br />Lane 2: Daudi cell lysate<br />Lane 3: Hela cell lysate<br />Lane 4: 293T cell lysate<br /><br />Predicted band size: 89 kDa<br />Observed band size: 120/50/55 kDa

    Western blot analysis of SF3A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500175, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

    Positive control:
    Lane 1: HL-60 cell lysate
    Lane 2: Daudi cell lysate
    Lane 3: Hela cell lysate
    Lane 4: 293T cell lysate

    Predicted band size: 89 kDa
    Observed band size: 120/50/55 kDa

  • Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-SF3A1 antibody (<a href="/products/HA500175" style="font-weight: bold;text-decoration: underline;">HA500175</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500175" style="font-weight: bold;text-decoration: underline;">HA500175</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-SF3A1 antibody (HA500175) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500175) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of Hela cells labeling SF3A1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA500175" style="font-weight: bold;text-decoration: underline;">HA500175</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Hela cells labeling SF3A1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA500175, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Immunohistochemical analysis of paraffin-embedded mouse brian tissue with Rabbit anti-SF3A1 antibody (<a href="/products/HA500175" style="font-weight: bold;text-decoration: underline;">HA500175</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500175" style="font-weight: bold;text-decoration: underline;">HA500175</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brian tissue with Rabbit anti-SF3A1 antibody (HA500175) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500175) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-SF3A1 antibody (<a href="/products/HA500175" style="font-weight: bold;text-decoration: underline;">HA500175</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500175" style="font-weight: bold;text-decoration: underline;">HA500175</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-SF3A1 antibody (HA500175) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500175) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"