EZH1 Rabbit Polyclonal Antibody
Catalog# HA500184
EZH1 Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
EZH1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human EZH1 aa 500-747.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
86/81/80/69 kDa
Positive Control
HepG2 cell lysate, mouse hippocampusl tissue lysate, rat brain tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
Target
Function
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation
Background References
1. Margueron R. et. al. Ezh1 and Ezh2 maintain repressive chromatin through different mechanisms. Mol. Cell 32:503-518(2008).
Subcellular Location
Nucleus.
Synonyms
Enhancer of zeste homolog 1 (Drosophila) antibody
Enhancer of zeste homolog 1 antibody
ENX-2 antibody
ENX2 antibody
Ezh1 antibody
EZH1_HUMAN antibody
Histone-lysine N-methyltransferase EZH1 antibody
Images
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Western blot analysis of EZH1 on different lysates with Rabbit anti-EZH1 antibody (HA500184) at 1/1,000 dilution.
Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate (15 µg/Lane)
Lane 2: Mouse hippocampus tissue lysate (30 µg/Lane)
Lane 3: Rat brain tissue lysate (30 µg/Lane)
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500184, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 85 kDa
Observed band size: 69-100 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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