Inhibin alpha Rabbit Polyclonal Antibody
Catalog# HA500185
Inhibin alpha Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Inhibin alpha Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human Inhibin alpha aa 240-290.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell
Target Molecular Weight
40 kDa
Positive Control
Rat testis tissue lysate, HepG2 cell lysate, mouse testis tissue lysate, PANC-1 cell lysate, PANC-1.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1000
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IF-Cell
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1:50-1:100
Target
Function
This gene encodes a member of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate multiple peptide products, including the alpha subunit of the inhibin A and B protein complexes. These complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion. Mutations in this gene may be associated with male infertility and premature ovarian failure in female human patients.
Background References
1. de Ávila MB. et. al. Structural Basis for Inhibition of Enoyl-[Acyl Carrier Protein] Reductase (InhA) from Mycobacterium tuberculosis. Curr Med Chem. 2020
2. Rodriguez F. et. al. First triclosan-based macrocyclic inhibitors of InhA enzyme. Bioorg Chem. 2020 Jan
Subcellular Location
Secreted.
Synonyms
A inhibin subunit antibody
A inhibin subunit precursor antibody
AW55578 antibody
IHA antibody
inhA antibody
INHA_HUMAN antibody
Inhibin alpha chain antibody
Inhibin alpha chain precursor antibody
Inhibin alpha subunit antibody
Images
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Western blot analysis of Inhibin alpha on different lysates with Rabbit anti-Inhibin alpha antibody (HA500185) at 1/1,000 dilution.
Lane 1: Mouse testis tissue lysate
Lane 2: Mouse ovary tissue lysate
Lane 3: Rat testis tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 135 seconds; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500185, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: multiple bands
Observed band size: multiple bands -
ICC staining of Inhibin alpha in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500185, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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