PLA2R Rabbit Polyclonal Antibody
Catalog# HA500209
PLA2R Rabbit Polyclonal Antibody
-
WB
-
IHC-P
-
IF-Tissue
-
Human
-
Mouse
-
unconjugated
Overview
Product Name
PLA2R Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human PLA2R aa 80-280.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 153 kDa
Positive Control
Present in lung macrophage (at protein level). Highly expressed in kidney. Also expressed in pancreas, amnion, choriodecidua and placenta. Isoform 2 is expressed at much lower level.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500-2000
-
IHC-P
-
1:100-500
-
IF-tissue
-
1:100-500
Target
Function
Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phospholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B.In podocytes, binding of sPLA2-IB/PLA2G1B can regulate podocyte survival and glomerular homeostasis.
Background References
1. Safar-Boueri L. et. al. Membranous nephropathy: diagnosis, treatment, and monitoring in the post-PLA2R era. Pediatr Nephrol. 2021 Jan
2. Burbelo PD. et. al. Detection of PLA2R Autoantibodies before the Diagnosis of Membranous Nephropathy. J Am Soc Nephrol. 2020 Jan
Subcellular Location
Cell membrane, Secreted.
Synonyms
180 kDa secretory phospholipase A2 receptor antibody
C-type lectin domain family 13 member C antibody
CLEC13C antibody
M type receptor antibody
M-type receptor antibody
Phospholipase A2 receptor 1 antibody
PLA2 R antibody
PLA2-R antibody
PLA2G1R antibody
PLA2IR antibody
ExpandImages
-
Sample:
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-PLA2R (HA500209) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 153 kD
Observed band size: 153 kD -
Paraformaldehyde-fixed, paraffin embedded (Human kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PLA2R) Polyclonal Antibody, Unconjugated (HA500209) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"