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STRAP / Unrip Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500221

STRAP / Unrip Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

STRAP / Unrip Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within C-terminal human STRAP from aa 200-350.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, FC

Molecular Weight

Predicted band size: 38 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, HepG2.

Conjugation

unconjugated

RRID

AB_3071318

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:200

  • FC

  • 1:1,000

Target

Function

The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. STRAP plays a role in the cellular distribution of the SMN complex. Negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein.

Background References

1. Valentino WL. et. al. The Utility of Strap Muscle in Complex Type I Thyroplasties. J Voice. 2019 Sep

2. de Vries AJ. et. al. Effect of a patellar strap on the joint position sense of the symptomatic knee in athletes with patellar tendinopathy. J Sci Med Sport. 2017 Nov

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q9Y3F4 Human

SwissProt: Q9Z1Z2 Mouse

SwissProt: Q5XIG8 Rat

Synonyms

MAP activator with WD repeats antibody

MAWD antibody

PTWD antibody

Serine-threonine kinase receptor-associated protein antibody

serine/threonine kinase receptor associated protein antibody

strap antibody

STRAP_HUMAN antibody

UNR-interacting protein antibody

UNRIP antibody

WD 40 repeat protein PT WD antibody

Expand

Images

  • Western blot analysis of STRAP / Unrip on different lysates with Rabbit anti-STRAP / Unrip antibody (<a href="/products/HA500221" style="font-weight: bold;text-decoration: underline;">HA500221</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate (10 µg/Lane)<br />Lane 2: HepG2 cell lysate (10 µg/Lane)<br />Lane 3: Mouse brain tissue lysate (20 µg/Lane)<br />Lane 4: Rat brain tissue lysate (20 µg/Lane)<br /><br />Predicted band size: 38 kDa<br />Observed band size: 38 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500221" style="font-weight: bold;text-decoration: underline;">HA500221</a>) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of STRAP / Unrip on different lysates with Rabbit anti-STRAP / Unrip antibody (HA500221) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate (10 µg/Lane)
    Lane 2: HepG2 cell lysate (10 µg/Lane)
    Lane 3: Mouse brain tissue lysate (20 µg/Lane)
    Lane 4: Rat brain tissue lysate (20 µg/Lane)

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500221) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HepG2 cells labeling STRAP / Unrip with Rabbit anti-STRAP / Unrip antibody (<a href="/products/HA500221" style="font-weight: bold;text-decoration: underline;">HA500221</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-STRAP / Unrip antibody (<a href="/products/HA500221" style="font-weight: bold;text-decoration: underline;">HA500221</a>) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HepG2 cells labeling STRAP / Unrip with Rabbit anti-STRAP / Unrip antibody (HA500221) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-STRAP / Unrip antibody (HA500221) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of HepG2 cells labeling STRAP / Unrip.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA500221" style="font-weight: bold;text-decoration: underline;">HA500221</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HepG2 cells labeling STRAP / Unrip.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA500221, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Alternative Products

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STRAP / Unrip Mouse Monoclonal Antibody [A7H11]

Application: WB,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated