RPE Rabbit Polyclonal Antibody
Catalog# HA500234
RPE Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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unconjugated
Overview
Product Name
RPE Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human RPE aa 50-228.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, IF-Cell
Molecular Weight
25 kDa
Positive Control
Mouse heart tissue lysate, human heart tissue lysate, human small intestine tissue, SH-SY5Y.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:2,000-5,000
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IHC-P
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1:500-1:5,000
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IF-Cell
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1:50-1:100
Target
Function
Phosphopentose epimerase (also known as ribulose-phosphate 3-epimerase and ribulose 5-phosphate 3-epimerase) encoded by the RPE gene is a metalloprotein that catalyzes the interconversion between D-ribulose 5-phosphate and D-xylulose 5-phosphate. This reversible conversion is required for carbon fixation in plants – through the Calvin cycle – and for the nonoxidative phase of the pentose phosphate pathway. This enzyme has also been implicated in additional pentose and glucuronate interconversions.
Background References
1. Le SB. et. al. 6-Phosphofructokinase and ribulose-5-phosphate 3-epimerase in methylotrophic Bacillus methanolicus ribulose monophosphate cycle. Appl Microbiol Biotechnol. 2017 May
2. Akana J. et. al. D-Ribulose 5-phosphate 3-epimerase: functional and structural relationships to members of the ribulose-phosphate binding (beta/alpha)8-barrel superfamily. Biochemistry. 2006 Feb
Subcellular Location
Cytosol, extracellular exosome.
Synonyms
2810429B02Rik antibody
5730518J08Rik antibody
EC 5.1.3.1 antibody
MGC124653 antibody
MGC2636 antibody
OTTHUMP00000163939 antibody
Ribulose 5 phosphate 3 epimerase antibody
Ribulose 5 phosphate epimerase antibody
Ribulose phosphate 3 epimerase antibody
Ribulose-5-phosphate-3-epimerase antibody
ExpandImages
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Western blot analysis of RPE on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500234, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse heart tissue lysate
Lane 2: Human heart tissue lysate -
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-RPE antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500234, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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ICC staining of RPE in SH-SY5Y cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500234, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"