SMG5 Rabbit Polyclonal Antibody
Catalog# HA500236
SMG5 Rabbit Polyclonal Antibody
-
WB
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
SMG5 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human SMG5 aa 800-1,000.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
114 kDa
Positive Control
Jurkat cell lysate, Hela cell lysate, 293 cell lysate, THP-1 cell lysate, mouse testis tissue lysates, rat testis tissue lysates, Hela.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:1,000-1:5,000
Target
Function
Plays a role in nonsense-mediated mRNA decay. Does not have RNase activity by itself. Promotes dephosphorylation of UPF1. Together with SMG7 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Necessary for TERT activity.
Background References
1. Nicholson P. et. al. Dissecting the functions of SMG5, SMG7, and PNRC2 in nonsense-mediated mRNA decay of human cells. RNA. 2018 Apr
2. Nelson JO. et. al. Multiple Nonsense-Mediated mRNA Processes Require Smg5 in Drosophila. Genetics. 2018 Aug
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
Protein SMG5
EST1-like protein B
LPTS-RP1
LPTS-interacting protein
SMG-5 homolog
hSMG-5
SMG5
EST1B
KIAA1089
EST1 like protein B antibody
ExpandImages
-
Western blot analysis of SMG5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500236, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Lane 3: 293 cell lysate
Lane 4: THP-1 cell lysate -
Western blot analysis of SMG5 on mouse testis tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500236, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
-
Western blot analysis of SMG5 on rat testis tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500236, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"