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ClC2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500243

ClC2 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

ClC2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human ClC2 aa 631-830.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, FC

Target Molecular Weight

Predicted band size: 99 kDa

Positive Control

HepG2 cell lysate, 293 cell lysate, SiHa, Hela.

Conjugation

unconjugated

RRID

AB_3071339

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500

  • IF-Cell

  • 1:200

  • FC

  • 1:500-1:1,000

Target

Function

This gene encodes a voltage-gated chloride channel. The encoded protein is a transmembrane protein that maintains chloride ion homeostasis in various cells. Defects in this gene may be a cause of certain epilepsies. Four transcript variants encoding different isoforms have been found for this gene. Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport. Involved in the regulation of aldosterone production. The opening of CLCN2 channels at hyperpolarized membrane potentials in the glomerulosa causes cell membrane depolarization, activation of voltage-gated Ca2+ channels and increased expression of aldosterone synthase, the rate-limiting enzyme for aldosterone biosynthesis.

Background References

1. Göppner C. et. al. Cellular basis of ClC-2 Cl(-) channel-related brain and testis pathologies. J Biol Chem. 2021 Jan-Jun

2. Ding W. et. al. ClC-2 inhibition prevents macrophage foam cell formation by suppressing Nlrp3 inflammasome activation. Biosci Biotechnol Biochem. 2020 Oct

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: P51788 Human

SwissProt: P35525 Rat

Synonyms

Chloride Channel 2 antibody

Chloride channel protein 2 antibody

Chloride channel, voltage sensitive 2 antibody

CIC 2 antibody

CIC2 antibody

ClC-2 antibody

CLC2 antibody

Clcn2 antibody

CLCN2_HUMAN antibody

ECA2 antibody

Expand

Images

  • Western blot analysis of ClC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA500243" style="font-weight: bold;text-decoration: underline;">HA500243</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: HepG2 cell lysate<br />Lane 2: 293 cell lysate<br /><br />Predicted band size: 99 kDa<br />Observed band size: 99 kDa

    Western blot analysis of ClC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500243, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: HepG2 cell lysate
    Lane 2: 293 cell lysate

    Predicted band size: 99 kDa
    Observed band size: 99 kDa

  • Immunocytochemistry analysis of SiHa cells labeling ClC2 with Rabbit anti-ClC2 antibody (<a href="/products/HA500243" style="font-weight: bold;text-decoration: underline;">HA500243</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ClC2 antibody (<a href="/products/HA500243" style="font-weight: bold;text-decoration: underline;">HA500243</a>) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of SiHa cells labeling ClC2 with Rabbit anti-ClC2 antibody (HA500243) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ClC2 antibody (HA500243) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Flow cytometric analysis of Hela cells labeling ClC2.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA500243" style="font-weight: bold;text-decoration: underline;">HA500243</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor&reg; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Hela cells labeling ClC2.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA500243, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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