Gankyrin Rabbit Polyclonal Antibody
Catalog# HA500244
Gankyrin Rabbit Polyclonal Antibody
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WB
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Human
-
unconjugated
Overview
Product Name
Gankyrin Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human Gankyrin aa 1-226.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 24 kDa
Positive Control
A549 cell lysate, Hela cell lysate, 293T cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500
Target
Function
This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.
Background References
1. Hao P. et. al. Inhibiting effect of MicroRNA-3619-5p/PSMD10 axis on liver cancer cell growth in vivo and in vitro. Life Sci. 2020 Aug
2. Wang C. et. al. LINC00665 Promotes the Progression of Multiple Myeloma by Adsorbing miR-214-3p and Positively Regulating the Expression of PSMD10 and ASF1B. Onco Targets Ther. 2020 Jul
Subcellular Location
Cytoplasm, Nucleus.
UNIPROT
Synonyms
26S proteasome non-ATPase regulatory subunit 10 antibody
26S proteasome regulatory subunit p28 antibody
Ankyrin repeat protein antibody
dJ889N15.2 antibody
Gankyrin antibody
Hepatocellular carcinoma-associated protein p28 II antibody
p28 antibody
p28(GANK) antibody
Proteasome (prosome, macropain) 26S subunit, non ATPase, 10 antibody
Proteasome 26S S10 antibody
ExpandImages
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Western blot analysis of Gankyrin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500244, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: Hela cell lysate
Lane 3: 293T cell lysate
Predicted band size: 24 kDa
Observed band size: 24 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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