Peptide YY Rabbit Polyclonal Antibody
Catalog# HA500250
Peptide YY Rabbit Polyclonal Antibody
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Peptide YY Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within N-terminal human Peptide YY.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 11 kDa
Positive Control
Human small intestine tissue lysate, RAW264.7 cell lysate, rat liver tissue lysate, SW620, SiHa.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:200
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FC
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1:100-1:500
Target
Function
Peptide YY (PYY) also known as peptide tyrosine tyrosine is a peptide that in humans is encoded by the PYY gene. Peptide YY is a short (36-amino acid) peptide released from cells in the ileum and colon in response to feeding. In the blood, gut, and other elements of periphery, PYY acts to reduce appetite; similarly, when injected directly into the central nervous system, PYY is also anorexigenic, i.e., it reduces appetite.Dietary fibers from fruits, vegetables, and whole grains, consumed, increase the speed of transit of intestinal chyme into the ileum, to raise PYY3-36, and induce satiety. Peptide YY can be produced as the result of enzymatic breakdown of crude fish proteins and ingested as a food product.
Background References
1. Tatemoto K. et. al. Isolation and primary structure of human peptide YY. Biochem. Biophys. Res. Commun. 157:713-717(1988).
Subcellular Location
Secreted.
Synonyms
GHYY antibody
MGC19143 antibody
Peptide tyrosine tyrosine antibody
peptide YY antibody
Peptide YY like antibody
Peptide YY precursor antibody
Peptide YY(3-36) antibody
Prepro PYY antibody
PYY 1 antibody
PYY antibody
ExpandImages
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Western blot analysis of Peptide YY on RAW264.7 (Mouse monocytic macrophage leukemia cell) lysates with Rabbit anti-Peptide YY antibody (HA500250) at 1/1,000 dilution.
Lysates/proteins at 15 µg/Lane.
Exposure time: 2 minutes 37 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500250, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 11 kDa
Observed band size: 20 kDa -
ICC staining of Peptide YY in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500250, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Flow cytometric analysis of Peptide YY was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500250, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"