MVD Rabbit Polyclonal Antibody
Catalog# HA500275
MVD Rabbit Polyclonal Antibody
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WB
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Human
-
Mouse
-
Rat
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unconjugated
Overview
Product Name
MVD Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human MVD aa 350-400 / 400.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 43 kDa
Positive Control
HEK-293 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Rat brain tissue lysate.
Conjugation
unconjugated
RRID
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | |
|---|---|
| human |
|
| mouse |
|
| rat |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
Target
Function
Diphosphomevalonate decarboxylase , most commonly referred to in scientific literature as mevalonate diphosphate decarboxylase.This enzyme converts mevalonate 5-diphosphate (MVAPP) to isopentenyl diphosphate (IPP) through ATP dependent decarboxylation.[1] The two substrates of this enzyme are ATP and mevalonate 5-diphosphate, whereas its 4 products are ADP, phosphate, isopentenyl diphosphate, and CO2.Mevalonate diphosphate decarboxylase catalyzes the final step in the mevalonate pathway. The mevalonate pathway is responsible for the biosynthesis of isoprenoids from acetate.[2] This pathway plays a key role in multiple cellular processes by synthesizing sterol isoprenoids, such as cholesterol, and non-sterol isoprenoids, such as dolichol, heme A, tRNA isopentenyltransferase, and ubiquinone.
Background References
1. Toth M.J. et. al. Molecular cloning and expression of the cDNAs encoding human and yeast mevalonate pyrophosphate decarboxylase. J. Biol. Chem. 271:7895-7898(1996).
Subcellular Location
Cytoplasm.
Synonyms
Diphosphomevalonate decarboxylase antibody
FP17780 antibody
MDDase antibody
Mevalonate (diphospho) decarboxylase antibody
Mevalonate (diphospho)decarboxylase antibody
Mevalonate pyrophosphate decarboxylase antibody
MPD antibody
Mvd antibody
MVD1_HUMAN antibody
Images
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Western blot analysis of MVD on different lysates with Rabbit anti-MVD antibody (HA500275) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: C6 cell lysate
Lane 5: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 43 kDa
Observed band size: 45 kDa
Exposure time: 51 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500275) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"