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Mitoferrin1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500278

Mitoferrin1 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

Mitoferrin1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human Mitoferrin1 aa 289-338.

Species Reactivity

Human, Mouse

Validated Applications

WB, IF-Cell, IHC-P

Target Molecular Weight

Predicted band size: 37 kDa

Positive Control

Mouse spleen tissue lysates, mouse brain tissue lysate, THP-1 cell lysate, Hela, mouse brain tissue.

Conjugation

unconjugated

RRID

AB_3073482

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500

  • IF-Cell

  • 1:50

  • IHC-P

  • 1:400

Target

Function

SLC25A37 is a solute carrier localized in the mitochondrial inner membrane. It functions as an essential iron importer for the synthesis of mitochondrial heme and iron-sulfur clusters (summary by Chen et al., 2009). Mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells, thereby playing an essential role in heme biosynthesis. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably delivered to ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX to make heme (By similarity).

Background References

1. Baldauf L. et. al. Mitoferrin-1 is required for brain energy metabolism and hippocampus-dependent memory. Neurosci Lett. 2019 Nov

2. Albayrak C. et. al. The role of hepcidin, GDF15, and mitoferrin-1 in iron metabolism of polycythemia vera and essential thrombocytosis patients. Turk J Med Sci. 2019 Feb

Subcellular Location

Mitochondrion inner membrane.

UNIPROT

SwissProt: Q9NYZ2 Human

SwissProt: Q920G8 Mouse

Synonyms

HT015 antibody

MFRN antibody

Mitochondrial iron transporter 1 antibody

Mitochondrial solute carrier protein antibody

Mitoferrin 1 antibody

MSC antibody

MSCP antibody

predicted protein of HQ2217 antibody

PRO1278 antibody

PRO1584 antibody

Expand

Images

  • Western blot analysis of Mitoferrin1 on mouse spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500278" style="font-weight: bold;text-decoration: underline;">HA500278</a>, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><br />Predicted band size: 37 kDa<br />Observed band size: 37 kDa

    Western blot analysis of Mitoferrin1 on mouse spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500278, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

  • Western blot analysis of Mitoferrin1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500278" style="font-weight: bold;text-decoration: underline;">HA500278</a>, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Mouse brain tissue lysate<br />Lane 2: THP-1 cell lysate<br /><br />Predicted band size: 37 kDa<br />Observed band size: 37 kDa

    Western blot analysis of Mitoferrin1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500278, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

    Positive control:
    Lane 1: Mouse brain tissue lysate
    Lane 2: THP-1 cell lysate

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

  • ICC staining of Mitoferrin1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/HA500278" style="font-weight: bold;text-decoration: underline;">HA500278</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Mitoferrin1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500278, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Mitoferrin1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500278" style="font-weight: bold;text-decoration: underline;">HA500278</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Mitoferrin1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500278, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"