Lck Rabbit Polyclonal Antibody
Catalog# HA500280
Lck Rabbit Polyclonal Antibody
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WB
-
FC
-
Human
-
Rat
-
unconjugated
Overview
Product Name
Lck Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human Lck aa 100-300 / 509.
Species Reactivity
Human, Rat
Validated Applications
WB, FC
Molecular Weight
58 kDa
Positive Control
Rat thymus tissue lysate, Jurkat cell lysate, Hela.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
-
FC
-
1:500-1:1,000
Target
Function
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described.
Background References
1. Bozso SJ. et. al. The role of competing mechanisms on Lck regulation. Immunol Res. 2020 Oct
2. Bommhardt U. et. al. Beyond TCR Signaling: Emerging Functions of Lck in Cancer and Immunotherapy. Int J Mol Sci. 2019 Jul
Subcellular Location
Cytosol, Cell membrane.
Synonyms
IMD22 antibody
LCK antibody
Lck p56 antibody
LCK proto-oncogene, Src family tyrosine kinase antibody
LCK_HUMAN antibody
Leukocyte C-terminal Src kinase antibody
LSK antibody
Lymphocyte cell specific protein tyrosine kinase antibody
Lymphocyte cell-specific protein-tyrosine kinase antibody
Lymphocyte specific protein tyrosine kinase antibody
ExpandImages
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Western blot analysis of Lck on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500280, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat thymus tissue lysate
Lane 2: Jurkat cell lysate -
Flow cytometric analysis of Lck was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500280, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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