IL-20R1 Rabbit Polyclonal Antibody
Catalog# HA500286
IL-20R1 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Human
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unconjugated
Overview
Product Name
IL-20R1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human IL-20R1 aa 471-520.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 62 kDa
Positive Control
MCF-7 cell lysate, SH-SY5Y cell lysate, SH-SY5Y, human kidney tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
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1:200
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IHC-P
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1:200
Target
Function
This gene encodes a member of the type II cytokine receptor family. The encoded protein is a subunit of the receptor for interleukin 20, a cytokine that may be involved in epidermal function. The interleukin 20 receptor is a heterodimeric complex consisting of the encoded protein and interleukin 20 receptor beta. This gene and interleukin 20 receptor beta are highly expressed in skin, and are upregulated in psoriasis. Alternative splicing results in multiple transcript variants.
Background References
1. Gao W. et. al. IL20RA signaling enhances stemness and promotes the formation of an immunosuppressive microenvironment in breast cancer. Theranostics. 2021 Jan
2. Ungaro F. et. al. mTOR-Dependent Stimulation of IL20RA Orchestrates Immune Cell Trafficking through Lymphatic Endothelium in Patients with Crohn\'s Disease. Cells. 2019 Aug
Subcellular Location
Membrane.
UNIPROT
Synonyms
class II cytokine receptor ZCYTOR7 antibody
CRF2 8 antibody
CRF2-8 antibody
Cytokine receptor class II member 8 antibody
Cytokine receptor class-II member 8 antibody
Cytokine receptor family 2 member 8 antibody
I20RA_HUMAN antibody
IL 20R alpha antibody
IL 20R1 antibody
IL-20 receptor subunit alpha antibody
ExpandImages
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Western blot analysis of IL-20R1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500286, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: SH-SY5Y cell lysate
Predicted band size: 62 kDa
Observed band size: 67 kDa (Glycosylation) -
ICC staining of IL-20R1 in SH-SY5Y cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500286, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IL-20R1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500286, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"