IL-21R Rabbit Polyclonal Antibody
Catalog# HA500290
IL-21R Rabbit Polyclonal Antibody
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WB
-
IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
IL-21R Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human IL-21R aa 51-100.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 59 kDa
Positive Control
Rat spleen tissue lysate, rat liver tissue lysate, rat thymus tissue lysate, mouse spleen tissue lysate, mouse thymus tissue lysate, Daudi cell lysate, human spleen tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IHC-P
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1:600
Target
Function
The protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). It belongs to the type I cytokine receptors, and has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2, 4, 7, 9, and 15. This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. Knockout studies of a similar gene in mouse suggest a role for this gene in regulating immunoglobulin production. Three alternatively spliced transcript variants have been described.
Background References
1. Bolzoni M. et. al. IL21R expressing CD14(+)CD16(+) monocytes expand in multiple myeloma patients leading to increased osteoclasts. Haematologica. 2017 Apr
2. Linnebacher A. et. al. Interleukin 21 Receptor/Ligand Interaction Is Linked to Disease Progression in Pancreatic Cancer. Cells. 2019 Sep
Subcellular Location
Membrane.
Synonyms
CD360 antibody
IL 21R antibody
IL-21 receptor antibody
IL-21R antibody
IL21 receptor antibody
IL21R antibody
IL21R_HUMAN antibody
Interleukin 21 receptor antibody
Interleukin-21 receptor antibody
MGC10967 antibody
ExpandImages
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Western blot analysis of IL-21R on different lysates with rabbit anti-IL-21R antibody (HA500290) at 1/500 dilution.
Lane 1: Rat spleen tissue lysate
Lane 2: Rat liver tissue lysate
Lane 3: Rat thymus tissue lysate
Lysates/proteins at 10 µg per lane.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500290, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Predicted band size: 59 kDa
Observed band size: 80 kDa (Glycosylation) -
Western blot analysis of IL-21R on different lysates with rabbit anti-IL-21R antibody (HA500290) at 1/500 dilution.
Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse thymus tissue lysate
Lane 3: Daudi cell lysate
Lysates/proteins at 10 µg per lane.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500290, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Predicted band size: 59 kDa
Observed band size: 80 kDa (Glycosylation) -
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL-21R antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500290, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"