HLA E Rabbit Polyclonal Antibody
Catalog# HA500299
HLA E Rabbit Polyclonal Antibody
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WB
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FC
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Human
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unconjugated
Overview
Product Name
HLA E Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human HLA E aa 22-200 / 358 (Extracellular).
Species Reactivity
Human
Validated Applications
WB, FC
Molecular Weight
40 kDa
Positive Control
Raji cell lysate, THP-1 cell lysate, HL-60 cell lysate, SiHa.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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FC
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1:100-1:500
Target
Function
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
Background References
1. Grant EJ. et. al. The unconventional role of HLA-E: The road less traveled. Mol Immunol. 2020 Apr
2. Kanevskiy L. et. al. Dimorphism of HLA-E and its Disease Association. Int J Mol Sci. 2019 Nov
Subcellular Location
Golgi apparatus membrane, Cell membrane, Secreted.
UNIPROT
Synonyms
HLA class I histocompatibility antigen E alpha chain antibody
EA1.2 antibody
EA2.1 antibody
HLA 6.2 antibody
HLA class I histocompatibility antigen alpha chain E antibody
HLA class I histocompatibility antigen E alpha chain precursor antibody
HLA class I histocompatibility antigen E alpha chain precursor antibody
HLA6.2 antibody
HLAE antibody
Lymphocyte antigen antibody
ExpandImages
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Western blot analysis of HLA E on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500299, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Raji cell lysate
Lane 2: THP-1 cell lysate
Lane 3: HL-60 cell lysate -
Flow cytometric analysis of HLA E was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500299, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"