CX3CL1 Rabbit Polyclonal Antibody
Catalog# HA500304
CX3CL1 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
CX3CL1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human CX3CL1 aa 25-341 / 397 (Extracellular).
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
42 kDa
Positive Control
SK-BR-3 cell lysate, HepG2 cell lysate, human skin tissue, human breast carcinoma tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:200
Target
Function
Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 . Binds to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 . The soluble form is chemotactic for T-cells and monocytes and not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium.
Background References
1. Pan Y. et. al. Neurotactin, a membrane-anchored chemokine upregulated in brain inflammation. Nature 387:611-617(1997).
Subcellular Location
Cell membrane, Membrane, Secreted.
UNIPROT
Synonyms
A 152E5.2 antibody
AB030188 antibody
ABCD 3 antibody
ABCD3 antibody
AI848747 antibody
C-X3-C motif chemokine 1 antibody
C3Xkine antibody
Chemokine (C-X3-C motif) ligand 1 antibody
Chemokine C X3 C motif ligand 1 antibody
Chemokine CX3C Motif Ligand 1 antibody
ExpandImages
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Western blot analysis of CX3CL1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500304, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SK-BR-3 cell lysate
Lane 2: HepG2 cell lysate -
Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CX3CL1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500304, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CX3CL1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500304, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"