ATP1B4 Rabbit Polyclonal Antibody
Catalog# HA500311
ATP1B4 Rabbit Polyclonal Antibody
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WB
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Mouse
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Rat
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Human
Predicted species support after-sales service
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unconjugated
Overview
Product Name
ATP1B4 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human ATP1B4 100-300 / 357.
Species Reactivity
Mouse, Rat (Predicted: Human)
Predicted species support after-sales service
Validated Applications
WB
Target Molecular Weight
Predicted band size: 42 kDa
Positive Control
Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate, Mouse heart tissue lysate, Human skeletal muscle tissue lysate, Mouse tongue tissue lysate, 293 cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
Target
Function
May act as a transcriptional coregulator during muscle development through its interaction with SNW1. Has lost its ancestral function as a Na,K-ATPase beta-subunit.
Background References
1. Pestov N.B. et. al. Evolution of Na,K-ATPase betam-subunit into a coregulator of transcription in placental mammals. Proc. Natl. Acad. Sci. U.S.A. 104:11215-11220(2007).
Subcellular Location
Membrane, Nucleus.
Synonyms
Protein ATP1B4 antibody
X,K-ATPase subunit beta-m antibody
X/potassium-transporting ATPase subunit beta-m antibody
ATP1B4 antibody
Images
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☑ Relative expression (RE)
Western blot analysis of ATP1B4 on different lysates with Rabbit anti-ATP1B4 antibody (HA500311) at 1/2,000 dilution.
Lane 1: Mouse skeletal muscle tissue lysate
Lane 2: Rat skeletal muscle tissue lysate
Lane 3: Mouse heart tissue lysate (low expression)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500311) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"