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Bcl2-L-13 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500329

Bcl2-L-13 Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Bcl2-L-13 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human Bcl2-L-13 aa 151-350/485.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

53 kDa

Positive Control

Hela cell lysate, Jurkat cell lysate, Raji cell lysate.

Conjugation

unconjugated

RRID

AB_3071424

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

Apoptosis is defined as a set of cascades which, when initiated, program the cell to undergo lethal changes such as membrane blebbing, mitochondrial breakdown and DNA fragmentation. The Bcl-2 family of proteins plays a central regulatory role in apoptosis. Bcl-rambo, a member of the Bcl-2 family, localizes to the mitochondria and, like other Bcl-2 family members, contains all four BH domains. Although Bcl-rambo shares structural similarity to other Bcl-2 members, it differs from them in its unique C-terminal region. Bcl-rambo has a 250 amino acid sequence containing two tandem repeats that preceeds the membrane anchor region at its C-terminus. Additionally, it is the membrane anchor C-terminal region of Bcl-rambo, not its Bcl-2 homology motifs, that is responsible for its pro-apoptotic activity. Bcl-rambo induces apoptosis when overexpressed and appears to do so by promoting mitochondrial cyto-chrome c release. It may also facilitate the activation of caspase-3.

Background References

1. Meng F. et. al. BCL2L13: physiological and pathological meanings. Cell Mol Life Sci. 2021 Mar

2. Lu Y. et. al. LncRNA FOXD3-AS1 knockdown protects against cerebral ischemia/reperfusion injury via miR-765/BCL2L13 axis. Biomed Pharmacother. 2020 Dec

Subcellular Location

Mitochondrion membrane, Nucleus.

UNIPROT

SwissProt: Q9BXK5 Human

Synonyms

Apoptosis facilitator antibody

B2L13_HUMAN antibody

Bcl 2 like 13 antibody

Bcl 2 like 13 protein antibody

Bcl rambo antibody

Bcl-2-like protein 13 antibody

Bcl-rambo antibody

BCL2 like 13 apoptosis facilitator antibody

BCL2 like 13 antibody

Bcl2 like 13 protein antibody

Expand

Images

  • Western blot analysis of Bcl2-L-13 on different lysates with Rabbit anti-Bcl2-L-13 antibody (<a href="/products/HA500329" style="font-weight: bold;text-decoration: underline;">HA500329</a>) at 1/500 dilution.<br /><br />Lane 1: Hela cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: Raji cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 53 kDa<br />Observed band size: 53 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500329" style="font-weight: bold;text-decoration: underline;">HA500329</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Bcl2-L-13 on different lysates with Rabbit anti-Bcl2-L-13 antibody (HA500329) at 1/500 dilution.

    Lane 1: Hela cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: Raji cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500329) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation