Wnt10a Rabbit Polyclonal Antibody (HA500356)
Catalog# HA500356
Rabbit Polyclonal to Wnt10a
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
Lysates/proteins at 10 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500356) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Wnt10a Rabbit Polyclonal Antibody (HA500356)
Immunogen
Recombinant protein within human wnt10a aa 201-417 / 417.
Host
Rabbit
Positive Control
RAW264.7 cell lysates, A375, human kidney tissue, mouse kidney tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500
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IF-Cell
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1:200
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IHC-P
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1:600
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 46 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Wnt10a
SYNONYMS
FLJ14301 antibody;OODD antibody;Protein Wnt-10a antibody;SSPS antibody;STHAG4 antibody;Wingless type MMTV integration site family member 10A antibody;WN10A_HUMAN antibody;wnt10a antibody
SUBCELLULAR LOCATION
Extracellular matrix, Secreted.
FUNCTION
Wnt-10a is a protein that in humans is encoded by the WNT10A gene. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. WNT10A is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines. The gene overexpression may play key roles in carcinogenesis through activation of the WNT-beta-catenin-TCF signaling pathway. This gene and the WNT6 gene are clustered in the chromosome 2q35 region. Mutations in the WNT10A gene are associated with Schöpf–Schulz–Passarge syndrome and hypodontia.
CITATIONS
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Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling
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IF
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WB
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Rat
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Citation
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