USP8 Rabbit Polyclonal Antibody
Catalog# HA500362
USP8 Rabbit Polyclonal Antibody
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WB
-
IHC-P
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Human
-
Mouse
-
Rat
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unconjugated
Overview
Product Name
USP8 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human USP8 aa 1-250.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 128 kDa
Positive Control
A375 cell lysate, A431 cell lysate, HepG2 cell lysate, 293T cell lysate, MCF-7 cell lysate, mouse testis tissue lysate, SK-Br-3 cell lysate, F9 cell lysate, rat lung tissue lysate, Hela cell lysate, mouse kidney tissue, human colon carcinoma tissue.
Conjugation
unconjugated
RRID
Reactivity Data
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
Product Features
Form
Liquid
Concentration
2.3 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
Target
Function
Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1. Deubiquitinates BACE1 which inhibits BACE1 lysosomal degradation and modulates BACE-mediated APP cleavage and amyloid-beta formation.
Background References
1. Yeates E.F., Tesco G. The Endosome-associated Deubiquitinating Enzyme USP8 Regulates BACE1 Enzyme Ubiquitination and Degradation. J. Biol. Chem. 291:15753-15766(2016) [PubMed] [Europe PMC]
2. Pohl C., Jentsch S. Final stages of cytokinesis and midbody ring formation are controlled by BRUCE. Cell 132:832-845(2008) [PubMed] [Europe PMC] [Abstract]
Subcellular Location
Cytoplasm, Cell membrane, Nucleus.
Synonyms
Deubiquitinating enzyme 8 antibody
FLJ34456 antibody
hUBPy antibody
HumORF8 antibody
KIAA0055 antibody
MGC129718 antibody
SPG59 antibody
Ubiquitin carboxyl-terminal hydrolase 8 antibody
Ubiquitin isopeptidase Y antibody
ubiquitin specific peptidase 8 antibody
ExpandImages
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Western blot analysis of USP8 on different lysates with Rabbit anti-USP8 antibody (HA500362) at 1/500 dilution.
Lane 1: A375 cell lysate (10 µg/Lane)
Lane 2: A431 cell lysate (10 µg/Lane)
Lane 3: HepG2 cell lysate (10 µg/Lane)
Lane 4: 293T cell lysate (10 µg/Lane)
Lane 5: MCF-7 cell lysate (10 µg/Lane)
Lane 6: Mouse testis tissue lysate (20 µg/Lane)
Lane 7: SK-Br-3 cell lysate (10 µg/Lane)
Lane 8: F9 cell lysate (10 µg/Lane)
Lane 9: Rat lung tissue lysate (20 µg/Lane)
Lane 10: Hela cell lysate (10 µg/Lane)
Predicted band size: 128 kDa
Observed band size: 128 kDa
Exposure time: 1 minute;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500362) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-USP8 antibody (HA500362) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500362) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-USP8 antibody (HA500362) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500362) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"