IFNGR2 Rabbit Polyclonal Antibody
Catalog# HA500363
IFNGR2 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Human
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Mouse
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
IFNGR2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human INGR2 22-71.
Species Reactivity
Human (Predicted: Mouse, Rat)
Predicted species support after-sales service
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 38 kDa
Positive Control
Jurkat cell lysate, Hela cell lysate, A431 cell lysate, human skeletal muscle tissue lysates, SH-SY5Y.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:200
Target
Function
Interferon gamma receptor 2 also known as IFN-γR2 is a protein which in humans is encoded by the IFNGR2 gene. This gene (IFNGR2) encodes the non-ligand-binding beta chain of the gamma interferon receptor. Human interferon-gamma receptor is a multimer of two IFN-γR1 chains (encoded by IFNGR1) and two IFN-γR2 chains. Associates with IFNGR1 to form a receptor for the cytokine interferon gamma (IFNG). Ligand binding stimulates activation of the JAK/STAT signaling pathway. Required for signal transduction in contrast to other receptor subunit responsible for ligand binding. Defects in IFNGR2 are a cause of autosomal recessive mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. All known mutations in IFNGR2 are collected in the IFNGR2 mutation database.
Background References
1. Kotenko S.V., Izotova L.S., Pollack B.P., Mariano T.M., Donnelly R.J., Muthukumaran G., Cook J.R., Garotta G., Silvennoinen O., Ihle J.N., et al. Interaction between the components of the interferon gamma receptor complex. J. Biol. Chem. 270:20915-20921(1995)
2. Rosenzweig S.D., Schwartz O.M., Brown M.R., Leto T.L., Holland S.M. Characterization of a dipeptide motif regulating IFN-gamma receptor 2 plasma membrane accumulation and IFN-gamma responsiveness. J. Immunol. 173:3991-3999(2004)
Subcellular Location
Endoplasmic reticulum, Plasma membrane, Golgi apparatus.
Synonyms
Interferon gamma receptor 2Imported antibody
IFN-gamma receptor 2 antibody
IFN-gamma-R2 antibody
Interferon gamma receptor accessory factor 1 antibody
AF-1 antibody
Interferon gamma receptor beta-chain antibody
IFN-gamma-R-beta antibody
Interferon gamma transducer 1 antibody
IFNGR2 antibody
IFNGT1 antibody
Images
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Western blot analysis of IFNGR2 on different lysates with Rabbit anti-IFNGR2 antibody (HA500363) at 1/500 dilution.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
Lane 3: A431 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 40 kDa
Exposure time: 1 minute;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500363) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of IFNGR2 on human skeletal muscle tissue lysates with Rabbit anti-IFNGR2 antibody (HA500363) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 40 kDa
Exposure time: 1 minute;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500363) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of SH-SY5Y cells labeling IFNGR2 with Rabbit anti-IFNGR2 antibody (HA500363) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IFNGR2 antibody (HA500363) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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