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alpha Tubulin Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500366

alpha Tubulin Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

  • Mouse

Predicted reactivity

  • Rat

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

alpha Tubulin Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human TBA3C aa 151-350 / 865.

Species Reactivity

Human, Mouse (Predicted: Rat)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IF-Cell

Molecular Weight

Predicted band size: 50 kDa

Positive Control

Hela cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, A431, SH-SY5Y, human testis tissue, mouse testis tissue.

Conjugation

unconjugated

RRID

AB_3071451

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IF-Cell

  • 1:200

Target

Function

Tubulin alpha-3C/D chain is a protein that in humans is encoded by the TUBA3C gene. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene is an alpha tubulin gene that encodes a protein 99% to the mouse testis-specific Tuba3 and Tuba7 gene products. This gene is located in the 13q11 region, which is associated with the genetic diseases Clouston hidrotic ectodermal dysplasia and Kabuki syndrome. Alternative splicing has been observed for this gene and two variants have been identified.

Background References

1. Dodé C, Weil D, Levilliers J, Crozet F, Chaïb H, Levi-Acobas F, Guilford P, Petit C (Jan 1998). "Sequence characterization of a newly identified human alpha-tubulin gene (TUBA2)". Genomics. 47 (1): 125–30.

Subcellular Location

Cytoskeleton

UNIPROT

SwissProt: P68366 Human

SwissProt: P68368 Mouse

SwissProt: Q5XIF6 Rat

Synonyms

Alpha-tubulin 1 antibody

ALS22 antibody

B ALPHA 1 antibody

bA408E5.3 antibody

H2 ALPHA antibody

Hum a tub1 antibody

Hum a tub2 antibody

LIS3 antibody

MGC171407 antibody

MGC55332 antibody

Expand

Images

  • Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (<a href="/products/HA500366" style="font-weight: bold;text-decoration: underline;">HA500366</a>) at 1/1,000 dilution.<br /><br />Lane 1: Hela cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: NIH/3T3 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 50 kDa<br />Observed band size: 55 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA500366" style="font-weight: bold;text-decoration: underline;">HA500366</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/1,000 dilution.

    Lane 1: Hela cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: NIH/3T3 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 50 kDa
    Observed band size: 55 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500366) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of A431 cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (<a href="/products/HA500366" style="font-weight: bold;text-decoration: underline;">HA500366</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (<a href="/products/HA500366" style="font-weight: bold;text-decoration: underline;">HA500366</a>) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of A431 cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry analysis of SH-SY5Y cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (<a href="/products/HA500366" style="font-weight: bold;text-decoration: underline;">HA500366</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (<a href="/products/HA500366" style="font-weight: bold;text-decoration: underline;">HA500366</a>) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of SH-SY5Y cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-alpha Tubulin antibody (<a href="/products/HA500366" style="font-weight: bold;text-decoration: underline;">HA500366</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500366" style="font-weight: bold;text-decoration: underline;">HA500366</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500366) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-alpha Tubulin antibody (<a href="/products/HA500366" style="font-weight: bold;text-decoration: underline;">HA500366</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500366" style="font-weight: bold;text-decoration: underline;">HA500366</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-alpha Tubulin antibody (HA500366) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500366) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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