ELKS Rabbit Polyclonal Antibody
Catalog# HA500376
ELKS Rabbit Polyclonal Antibody
-
WB
-
IHC-P
-
IF-Cell
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
ELKS Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human ELKS aa 850-1,116.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell
Target Molecular Weight
Predicted band size: 128 kDa
Positive Control
HEK-293 cell lysate, A549 cell lysate, SiHa cell lysate, NIH/3T3 cell lysate, rat testis tissue, human liver tissue, human kidney tissue, human placenta tissue, mouse large intestine tissue, A375, SiHa.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
2.39 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:5000
-
IHC-P
-
1:800
-
IF-Cell
-
1:200
Target
Function
ELKS/RAB6-interacting/CAST family member 1 is a protein that in humans is encoded by the ERC1 gene. The name ELKS is derived from "protein rich in the amino acids E, L, K and S". The protein encoded by this gene is a member of a family of RIM-binding proteins. RIMs are active zone proteins that regulate neurotransmitter release. This gene has been found fused to the receptor-type tyrosine kinase gene RET by gene rearrangement due to the translocation t(10;12)(q11;p13). Multiple transcript variants encoding different isoforms have been found for this gene. ELKS has been reported to direct vesicles with RAB6A to melanosomes. Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex. May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport. Widely expressed. Isoform 2 and isoform 4 are abundantly expressed in brain. Isoform 1 and isoform 3 are predominantly expressed in testis and thyroid, and isoform 1 predominates in other tissues tested.
Background References
1. Ducut Sigala J.L., Bottero V., Young D.B., Shevchenko A., Mercurio F., Verma I.M. Activation of transcription factor NF-kappaB requires ELKS, an IkappaB kinase regulatory subunit. Science 304:1963-1967(2004).
2. Nakata T., Yokota T., Emi M., Minami S. Differential expression of multiple isoforms of the ELKS mRNAs involved in a papillary thyroid carcinoma. Genes Chromosomes Cancer 35:30-37(2002).
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosomem, Cytoplasm, Membrane, Golgi apparatus membrane, Cell junction, synapse, presynaptic cell membrane.
Synonyms
Cast 2 antibody
CAST2 antibody
CAZ associated structural protein 2 antibody
Elks antibody
ELKS/RAB6 interacting/CAST family member 1 antibody
ELKS/RAB6-interacting/CAST family member 1 antibody
ERC 1 antibody
ERC protein 1 antibody
ERC-1 antibody
Erc1 antibody
ExpandImages
-
Western blot analysis of ELKS on different lysates with Rabbit anti-ELKS antibody (HA500376) at 1/5,000 dilution.
Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate
Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 3: SiHa (Human cervical cancersSquamous cell) cell lysate
Lane 4: NIH/3T3 (Mouse fibroblast) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 59 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500376, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 128 kDa
Observed band size: 128 kDa -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-ELKS antibody (HA500376) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500376) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ELKS antibody (HA500376) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500376) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ELKS antibody (HA500376) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500376) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-ELKS antibody (HA500376) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500376) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue with Rabbit anti-ELKS antibody (HA500376) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500376) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of A375 cells labeling ELKS with Rabbit anti-ELKS antibody (HA500376) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ELKS antibody (HA500376) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Immunocytochemistry analysis of SiHa cells labeling ELKS with Rabbit anti-ELKS antibody (HA500376) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ELKS antibody (HA500376) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
ELKS Recombinant Rabbit Monoclonal Antibody [PSH17-33]
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway
ELKS Recombinant Rabbit Monoclonal Antibody [PSH17-33] - BSA and Azide free
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated