Histone H2B Rabbit Polyclonal Antibody
Catalog# HA500381
Histone H2B Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
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Zebrafish
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unconjugated
Overview
Product Name
Histone H2B Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within C-terminal human Histone H2B.
Species Reactivity
Human, Mouse, Rat, Zebrafish
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 14 kDa
Positive Control
MCF-7 cell lysate, Hela cell lysate, A549 cell lysate, 293T cell lysate, THP-1 cell lysate, K562 cell lysate, HL-60 cell lysate, NIH/3T3 cell lysate, zebrafish tissue lysate, PC-12 cell lysate, RAW264.7 cell lysate, rat large intestine tissue, rat liver tissue, human breast tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1.7 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:2,000
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IHC-P
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1:5,000
Target
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.
Background References
暂无
Subcellular Location
Nucleus
UNIPROT
Synonyms
GL105 antibody
H2B GL105 antibody
H2B histone family member O antibody
H2B histone family member S antibody
H2B.1 antibody
H2B.1 B antibody
H2B.b antibody
H2B.c antibody
H2B.d antibody
H2B.e antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-Histone H2B antibody (HA500381) at 1/3,400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500381) at 1/3,400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Histone H2B antibody (HA500381) at 1/3,400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500381) at 1/3,400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Histone H2B antibody (HA500381) at 1/3,400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500381) at 1/3,400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of Histone H2B on different lysates with Rabbit anti-Histone H2B antibody (HA500381) at 1/1,000 dilution.
Lane 1: MCF-7 (Human breast cancer cell) cell lysate
Lane 2: Hela (Human cervical adenocarcinoma cell) cell lysate
Lane 3: 293T (Human embryonic kidney cell) cell lysate
Lane 4：THP-1 (Human acute monoblastic leukemia cell) cell lysate
Lane 5: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
Lane 6: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate
Lane 7: NIH/3T3 (Mouse fibroblast) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 6 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500381, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 14 kDa
Observed band size: 14 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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