Histone H2B Rabbit Polyclonal Antibody
Catalog# HA500381
Histone H2B Rabbit Polyclonal Antibody
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WB
-
IHC-P
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Human
-
Mouse
-
Rat
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Zebrafish
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unconjugated
Overview
Product Name
Histone H2B Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within C-terminal human Histone H2B.
Species Reactivity
Human, Mouse, Rat, Zebrafish
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 14 kDa
Positive Control
MCF-7 cell lysate, Hela cell lysate, A549 cell lysate, 293T cell lysate, THP-1 cell lysate, K562 cell lysate, HL-60 cell lysate, NIH/3T3 cell lysate, zebrafish tissue lysate, PC-12 cell lysate, RAW264.7 cell lysate, rat large intestine tissue, rat liver tissue, human breast tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:2,000
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IHC-P
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1:5,000
Target
Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Post-translational modification Monoubiquitination at Lys-35 (H2BK34Ub) by the MSL1/MSL2 dimer is required for histone H3 'Lys-4' (H3K4me) and 'Lys-79' (H3K79me) methylation and transcription activation at specific gene loci, such as HOXA9 and MEIS1 loci. Similarly, monoubiquitination at Lys-121 (H2BK120Ub) by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. H2BK120Ub also acts as a regulator of mRNA splicing: deubiquitination by USP49 is required for efficient cotranscriptional splicing of a large set of exons.
Background References
暂无
Subcellular Location
Nucleus
UNIPROT
Synonyms
GL105 antibody
H2B GL105 antibody
H2B histone family member O antibody
H2B histone family member S antibody
H2B.1 antibody
H2B.1 B antibody
H2B.b antibody
H2B.c antibody
H2B.d antibody
H2B.e antibody
ExpandImages
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Western blot analysis of Histone H2B on different lysates with Rabbit anti-Histone H2B antibody (HA500381) at 1/2,000 dilution.
Lane 1: MCF-7 cell lysate (10 µg/Lane)
Lane 2: Hela cell lysate (10 µg/Lane)
Lane 3: A549 cell lysate (10 µg/Lane)
Lane 4: 293T cell lysate (10 µg/Lane)
Lane 5: THP-1 cell lysate (10 µg/Lane)
Lane 6: K562 cell lysate (10 µg/Lane)
Lane 7: HL-60 cell lysate (10 µg/Lane)
Lane 8: NIH/3T3 cell lysate (10 µg/Lane)
Lane 9: Zebrafish tissue lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (10 µg/Lane)
Lane 11: RAW264.7 cell lysate (10 µg/Lane)
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 1 minute;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500381) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-Histone H2B antibody (HA500381) at 1/3,400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500381) at 1/3,400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Histone H2B antibody (HA500381) at 1/3,400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500381) at 1/3,400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Histone H2B antibody (HA500381) at 1/3,400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500381) at 1/3,400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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