PGBD5 Rabbit Polyclonal Antibody
Catalog# HA500382
PGBD5 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
PGBD5 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human PGBD5 aa 270-520.
Species Reactivity
Human, Mouse (Predicted: Rat)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 58 kDa
Positive Control
Daudi cell lysates, human lung carcinoma tissue, mouse uterus tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
Target
Function
PiggyBac Transposable Element Derived 5 is an enzyme that in humans is encoded by the PGBD5 gene. The putative catalytic triad of the protein composed of three aspartic acid residues is conserved among PGBD5-like genes through evolution, and is distinct from other PiggyBac-like genes. PGBD5 has been shown to be able to transpose DNA in a sequence-specific, cut-and-paste fashion. PGBD5 has also been proposed to mediate site-specific DNA rearrangements in human tumors. Human PGBD5 can mobilize the insect PiggyBac transposons in human cell culture. In mature mice brain tissue PGBD5 is found primarily in regions of the olfactory bulb, hippocampus, and cerebellum. In embryonic mice brain tissue PGBD5 is found not only in the medial pallium and prepontine isthmus, which are embryonic brain areas that give rise to the development of the hippocampus and cerebellum but also in areas in the embryonic brain that give rise to the hypothalamus and medulla. PGBD5 is expressed in the majority of human pediatric solid tumors. It's upregulated in sporadic Creutzfeldt-Jakob disease. PGBD5 is associated with frontotemporal dementia, where it gets most expressed in neurons, followed by ogliodendrocytes, mature astrocytes, fetal astrocytes, endothelial cells and then microglia/macrophages.
Background References
1. Henssen A.G., Koche R., Zhuang J., Jiang E., Reed C., Eisenberg A., Still E., MacArthur I.C., Rodriguez-Fos E., Gonzalez S., Puiggros M., Blackford A.N., Mason C.E., de Stanchina E., Goenen M., Emde A.K., Shah M., Arora K. PGBD5 promotes site-specific oncogenic mutations in human tumors. Nat. Genet. 49:1005-1014(2017).
2. Henssen A.G., Jiang E., Zhuang J., Pinello L., Socci N.D., Koche R., Gonen M., Villasante C.M., Armstrong S.A., Bauer D.E., Weng Z., Kentsis A. Forward genetic screen of human transposase genomic rearrangements. BMC Genomics 17:548-548(2016).
Subcellular Location
Nucleus.
Synonyms
PGBD5 antibody
PGBD5_HUMAN antibody
PiggyBac transposable element-derived protein 5 antibody
Images
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Western blot analysis of PGBD5 on Daudi cell lysates with Rabbit anti-PGBD5 antibody (HA500382) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500382) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PGBD5 antibody (HA500382) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500382) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse uterus tissue with Rabbit anti-PGBD5 antibody (HA500382) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500382) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"