IL23R Rabbit Polyclonal Antibody
Catalog# HA500387
IL23R Rabbit Polyclonal Antibody
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WB
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IF-Cell
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Human
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unconjugated
Overview
Product Name
IL23R Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human IL23R aa 440-490.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Target Molecular Weight
Predicted band size: 72 kDa
Positive Control
MCF-7 cell lysate, 293 cell lysate, SH-SY5Y.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1.88 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:200
Target
Function
The interleukin-23 receptor is a type I cytokine receptor. It is encoded in human by the IL23R gene. In complex with the interleukin-12 receptor β1 subunit (IL-12Rβ1), it is activated by the cytokine interleukin 23 (IL-23). The IL23R mRNA is 2.8 kilobases in length and includes 12 exons. The translated protein contains 629 amino acids; it is a type I penetrating protein and includes a signal peptide, an N-terminal fibronectin III-like domain and an intracellular part that contains three potential tyrosine phosphorylation domains. There are 24 IL23R splice variants in mitogen-activated lymphocytes. IL23R includes some single-nucleotide polymorphisms in the region encoding the domain that binds IL-23, which may lead to differences between people in Th17 activation. There is also a variant of IL-23R that consists of just the extracellular part and is known as soluble IL-23R. This form can compete with the membrane-bound form to bind IL-23, modulating the Th17 immune response and regulation of inflammation and immune function. The protein encoded by this gene is a subunit of the receptor for IL-23. This protein pairs with the receptor molecule IL-12Rβ1 (IL12RB1), together forming the IL-23 receptor complex, and both are required for IL-23 signaling. This protein associates constitutively with Janus kinase 2 (JAK2) and also binds to transcription activator STAT3 in a ligand-dependent manner. Three variants in the IL23R gene have been shown to protect against Crohn's disease and ulcerative colitis. The effect of IL-23R variations present in the population have been studied with an in vitro expression model system.
Background References
1. Cui Y et al. IL23R-Specific CAR Tregs for the Treatment of Crohn\'s Disease. J Crohns Colitis. 2025 Mar
2. Zhu X et al. Intermittent Fasting-Induced Orm2 Promotes Adipose Browning via the GP130/IL23R-p38 Cascade. Adv Sci (Weinh). 2024 Nov
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
IL 23R antibody
IL-23 receptor antibody
IL-23R antibody
IL23 Receptor antibody
Il23r antibody
IL23R_HUMAN antibody
Interleukin 23 receptor antibody
Interleukin-23 receptor antibody
Images
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Western blot analysis of IL23R on different lysates with Rabbit anti-IL23R antibody (HA500387) at 1/500 dilution.
Lane 1: MCF-7 cell lysate
Lane 2: 293 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 72 kDa
Observed band size: 43 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500387) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of SH-SY5Y cells labeling IL23R with Rabbit anti-IL23R antibody (HA500387) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IL23R antibody (HA500387) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"