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SOX3 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500388

SOX3 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

SOX3 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human SOX3 aa 20-70.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 45 kDa

Positive Control

MCF-7 cell lysate, A549 cell lysate, Hela cell lysate, human cerebellum tissue lysate, mouse cerebellum tissue lysate, rat cerebellum tissue lysate, human testis tissue, human colon tissue, human esophagus tissue, human fallopian tube tissue.

Conjugation

unconjugated

RRID

AB_3071473

Reactivity Data

WB IHC-P
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

2.3 mg/ml(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000-5,000

  • IHC-P

  • 1:600

Target

Function

Transcription factor SOX-3 is a protein that in humans is encoded by the SOX3 gene. This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic brain development and in determination of cell fate. The encoded protein acts as a transcriptional activator. Mutations in this gene have been associated with X-linked hypopituitarism (XH) and X-linked mental retardation. Patients with XH are male, have short stature, exhibit a mild form of mental retardation and present pan-hypopituitarism. A duplication of the SOX3 gene has also been discovered to cause XX male sex reversal. SRY-box transcription factor 3, SOX3, is a transcription factor that is encoded by the SOX3 gene. This gene is responsible for ensuring proper embryonic development and determining the fate of different cells. Regarding its developmental facet, SOX3, alongside other SOX transcription factors, ensures the proper formation of the hypothalamo-pituitary axis. The proper development of the hypothalamo-pituitary axis is necessary as it serves to ensure proper systemic hormonal function. When SOX3 expression is affected, the development of different structures can be affected as well. Specifically, both the hypothalamus and the pituitary gland can suffer in accomplishing proper growth. Due to this, conditions such as hypopituitarism and mental retardation are found in cases with a lack of SOX3. Also, craniofacial abnormalities can be seen as a result of a lack of the SOX3 gene.

Background References

1. Barber, TM, Cheetham T, Ball SG (2004). X-linked hypopituitarism: clinical and biochemical features of a rare cause of short stature. Endocrine Abstracts. 7 (1): 248.

2. Moalem S, Babul-Hirji R, Stavropolous DJ, Wherrett D, Bägli DJ, Thomas P, Chitayat D (July 2012). XX male sex reversal with genital abnormalities associated with a de novo SOX3 gene duplication. American Journal of Medical Genetics. Part A. 158A (7): 1759–64.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P41225 Human

SwissProt: P53784 Mouse

Entrez Gene: 679158 Rat

Synonyms

GHDX antibody

MRGH antibody

PHP antibody

PHPX antibody

sox3 antibody

SOX3_HUMAN antibody

SOXB antibody

SRY (sex determining region Y)-box 3 antibody

SRY Box 3 antibody

SRY-related HMG-box gene 3 antibody

Expand

Images

  • Western blot analysis of SOX3 on different lysates with Rabbit anti-SOX3 antibody (<a href="/products/HA500388" style="font-weight: bold;text-decoration: underline;">HA500388</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell)(15 µg/Lane)<br />Lane 2: MCF7 (Human breast cancer cell)(15 µg/Lane)<br />Lane 3: A549 (Human lung adenocarcinoma cell)(15 µg/Lane)<br />Lane 4: Mouse cerebellum tissue lysate(30 µg/Lane)<br />Lane 5: Rat cerebellum tissue lysate(30 µg/Lane)<br /><br />Exposure time: 60 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500388" style="font-weight: bold;text-decoration: underline;">HA500388</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 45 kDa<br />Observed band size: 38 kDa

    Western blot analysis of SOX3 on different lysates with Rabbit anti-SOX3 antibody (HA500388) at 1/5,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell)(15 µg/Lane)
    Lane 2: MCF7 (Human breast cancer cell)(15 µg/Lane)
    Lane 3: A549 (Human lung adenocarcinoma cell)(15 µg/Lane)
    Lane 4: Mouse cerebellum tissue lysate(30 µg/Lane)
    Lane 5: Rat cerebellum tissue lysate(30 µg/Lane)

    Exposure time: 60 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500388, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 45 kDa
    Observed band size: 38 kDa

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SOX3 antibody (<a href="/products/HA500388" style="font-weight: bold;text-decoration: underline;">HA500388</a>) at 1/600 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500388" style="font-weight: bold;text-decoration: underline;">HA500388</a>) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SOX3 antibody (HA500388) at 1/600 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500388) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-SOX3 antibody (<a href="/products/HA500388" style="font-weight: bold;text-decoration: underline;">HA500388</a>) at 1/600 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500388" style="font-weight: bold;text-decoration: underline;">HA500388</a>) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-SOX3 antibody (HA500388) at 1/600 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500388) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-SOX3 antibody (<a href="/products/HA500388" style="font-weight: bold;text-decoration: underline;">HA500388</a>) at 1/600 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500388" style="font-weight: bold;text-decoration: underline;">HA500388</a>) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-SOX3 antibody (HA500388) at 1/600 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500388) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-SOX3 antibody (<a href="/products/HA500388" style="font-weight: bold;text-decoration: underline;">HA500388</a>) at 1/600 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500388" style="font-weight: bold;text-decoration: underline;">HA500388</a>) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue with Rabbit anti-SOX3 antibody (HA500388) at 1/600 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500388) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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