Cathepsin Z Rabbit Polyclonal Antibody
Catalog# HA500396
Cathepsin Z Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Mouse
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unconjugated
Overview
Product Name
Cathepsin Z Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human Cathepsin Z aa 62-303.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P, IF-Cell, FC
Target Molecular Weight
Predicted band size: 34 kDa
Positive Control
Human kidney tissue lysate, Hela cell lysate, human liver tissue, mouse liver tissue, HeLa, SiHa.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
2.05 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:500-1:1,000
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IF-Cell
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1:100
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FC
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1:1,000
Target
Function
Cathepsin Z, also called cathepsin X or cathepsin P, is a protein that in humans is encoded by the CTSZ gene. It is a member of the cysteine cathepsin family of cysteine proteases, which has 11 members. As one of the 11 cathepsins, cathepsin Z contains distinctive features from others. Cathepsin Z has been reported involved in cancer malignancy and inflammation. This gene is expressed ubiquitously in cancer cell lines and primary tumors and, like other members of this family, may be involved in tumorigenesis. For instance, cathepsin Z promotes invasion and migration via a noncatalytic mechanism, suggesting multiple modes of cell invasion may be involved in cancer malignancy. Cathepsin Z is also reported to have a protective, but not proteolytic, function in inflammatory gastric disease. It is reported in another study that cathepsin Z may be responsible for dopamine neuron death and thus involved in the pathogenic cascade event. Single-nucleotide polymorphism in CTSZ is found associated with tuberculosis susceptibility, indicating that the pathways involving this protein could yield novel therapies for tuberculosis.
Background References
1. Kos J, Jevnikar Z, Obermajer N (April–June 2009). "The role of cathepsin X in cell signaling". Cell Adhesion & Migration. 3 (2): 164–6.
2. Obermajer N, Svajger U, Bogyo M, Jeras M, Kos J (November 2008). "Maturation of dendritic cells depends on proteolytic cleavage by cathepsin X". Journal of Leukocyte Biology. 84 (5): 1306–15.
Subcellular Location
Lysosome
Synonyms
Carboxypeptidase LB antibody
Cathepsin B2 antibody
Cathepsin IV antibody
Cathepsin P antibody
Cathepsin X antibody
Cathepsin X precursor antibody
Cathepsin Y antibody
Cathepsin Z antibody
Cathepsin Z precursor antibody
Cathepsin Z1 antibody
ExpandImages
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Western blot analysis of Cathepsin Z on different lysates with Rabbit anti-Cathepsin Z antibody (HA500396) at 1/1,000 dilution.
Lane 1: Human kidney tissue lysate (20 µg/Lane)
Lane 2: Hela cell lysate (10 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 36 kDa
Exposure time: 1 minute;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500396) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Cathepsin Z antibody (HA500396) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500396) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Cathepsin Z antibody (HA500396) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500396) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of HeLa cells labeling Cathepsin Z with Rabbit anti-Cathepsin Z antibody (HA500396) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cathepsin Z antibody (HA500396) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of SiHa cells labeling Cathepsin Z with Rabbit anti-Cathepsin Z antibody (HA500396) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cathepsin Z antibody (HA500396) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HeLa cells labeling Cathepsin Z.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500396, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"