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Tim23 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500398

Tim23 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Tim23 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human Tim23 aa 30-80.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IF-Cell

Molecular Weight

Predicted band size: 22 kDa

Positive Control

Hela cell lysate, human kidney tissue, mouse colon tissue, A549.

Conjugation

unconjugated

RRID

AB_3071483

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:800

  • IF-Cell

  • 1:200

Target

Function

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. The vast majority of mitochondrial proteins are encoded by nuclear genes, synthesized in the cytosol and imported into the organelle by a multi-protein import complex. The major mitochondrial import pathway transports preproteins with cleavable N-terminal presequences. The preproteins are recognized by the receptors TOMM20 and TOMM22 of the translocase of the outer mitochondrial membrane (TOM complex) and handed over from the TOM complex to the presequence translocase of the inner mitochondrial membrane (TIM23 complex) via a two-membrane-spanning super-complex intermediate. Humans have two TIM23 orthologs. TIMM23 codes for a single protein, TIMM23, which functions as part of the TIM23 complex. TIMM23B was identified by massive sequencing and codes for two proteins, TIMM23B1 and TIMM23B2, which are highly similar to TIMM23

Background References

1. Jesús A.Prieto-Ruiz et al. Expression of the human TIMM23 and TIMM23B genes is regulated by the GABP transcription factor. Volume 1861, Issue 2, February 2018, Pages 80-94.

Subcellular Location

Mitochondrion inner membrane.

UNIPROT

SwissProt: O14925 Human

SwissProt: Q9WTQ8 Mouse

Synonyms

MGC22767 antibody

MGC93478 antibody

Mitochondrial import inner membrane translocase subunit Tim23 antibody

Predicted protein of HQ1197 antibody

PRO1197 antibody

TIM23 antibody

TIMM 23 antibody

TIMM23 antibody

Translocase of inner mitochondrial membrane 23 (yeast) homolog antibody

Translocase of inner mitochondrial membrane 23 homolog (yeast) antibody

Expand

Images

  • Western blot analysis of Tim23 on Hela cell lysate with Rabbit anti-Tim23 antibody (<a href="/products/HA500398" style="font-weight: bold;text-decoration: underline;">HA500398</a>) at 1/5,000 dilution.<br /><br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500398" style="font-weight: bold;text-decoration: underline;">HA500398</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 21.9 kDa<br />Observed band size: 22 kDa

    Western blot analysis of Tim23 on Hela cell lysate with Rabbit anti-Tim23 antibody (HA500398) at 1/5,000 dilution.


    Lysates/proteins at 10 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500398, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 21.9 kDa
    Observed band size: 22 kDa

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Tim23 antibody (<a href="/products/HA500398" style="font-weight: bold;text-decoration: underline;">HA500398</a>) at 1/800 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500398" style="font-weight: bold;text-decoration: underline;">HA500398</a>) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Tim23 antibody (HA500398) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500398) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Tim23 antibody (<a href="/products/HA500398" style="font-weight: bold;text-decoration: underline;">HA500398</a>) at 1/800 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500398" style="font-weight: bold;text-decoration: underline;">HA500398</a>) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Tim23 antibody (HA500398) at 1/800 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500398) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry analysis of A549 cells labeling Tim23 with Rabbit anti-Tim23 antibody (<a href="/products/HA500398" style="font-weight: bold;text-decoration: underline;">HA500398</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tim23 antibody (<a href="/products/HA500398" style="font-weight: bold;text-decoration: underline;">HA500398</a>) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 647, <a href="/products/HA1127" style="font-weight: bold;text-decoration: underline;">HA1127</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of A549 cells labeling Tim23 with Rabbit anti-Tim23 antibody (HA500398) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tim23 antibody (HA500398) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation