UBE2I Rabbit Polyclonal Antibody
Catalog# HA500401
UBE2I Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
-
Rat
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unconjugated
Overview
Product Name
UBE2I Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human UBE2I aa 2-158.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 18 kDa
Positive Control
HepG2 cell lysate, 293T cell lysate, Hela cell lysate, Jurkat cell lysate, PC-12 cell lysates.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:500-1:1,000
Target
Function
Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity. This protein is involved in the pathway protein sumoylation, which is part of Protein modification. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Background References
1. Liang Y.C., Lee C.C., Yao Y.L., Lai C.C., Schmitz M.L., Yang W.M. SUMO5, a novel poly-sumo isoform, regulates pml nuclear bodies. Sci. Rep. 6:26509-26509(2016)
2. Cappadocia L., Pichler A., Lima C.D. Structural basis for catalytic activation by the human ZNF451 SUMO E3 ligase. Nat. Struct. Mol. Biol. 22:968-975(2015)
Subcellular Location
Cytoplasm. Nucleus.
Synonyms
C358B7.1 antibody
p18 antibody
SUMO 1 protein ligase antibody
SUMO conjugating enzyme UBC9 antibody
SUMO-conjugating enzyme UBC9 antibody
SUMO-protein ligase antibody
SUMO1 protein ligase antibody
UBC9 antibody
UBC9_HUMAN antibody
UBCE9 antibody
ExpandImages
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Western blot analysis of UBE2I on different lysates with Rabbit anti-UBE2I antibody (HA500401) at 1/500 dilution.
Lane 1: HepG2 cell lysate
Lane 2: 293T cell lysate
Lane 3: Hela cell lysate
Lane 4: Jurkat cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 1 minute;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500401) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of UBE2I on PC-12 cell lysates with Rabbit anti-UBE2I antibody (HA500401) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500401) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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