FAB1A Rabbit Polyclonal Antibody
Catalog# HA500403
FAB1A Rabbit Polyclonal Antibody
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IF-Tissue
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IHC-P
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Arabidopsis thaliana
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unconjugated
Overview
Product Name
FAB1A Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Arabidopsis thaliana FAB1A aa 100-200 / 1757.
Species Reactivity
Arabidopsis thaliana
Validated Applications
IF-Tissue, IHC-P
Target Molecular Weight
196 kDa
Positive Control
A. thaliana tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 1% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IF-Tissue
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1:50-1:100
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IHC-P
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1:50-1:200
Target
Function
The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate (By similarity). Plays an important role in maintenance of endomembrane homeostasis including endocytosis, vacuole formation, and vacuolar acidification processes. Required for development of viable pollen. Might mediate recycling of auxin transporters.
Background References
1. Hirano T. et. al. Loss-of-function and gain-of-function mutations in FAB1A/B impair endomembrane homeostasis, conferring pleiotropic developmental abnormalities in Arabidopsis. Plant Physiol. 155:797-807(2011).
2. Hirano T. et. al. Arabidopsis FAB1A/B is possibly involved in the recycling of auxin transporters. Plant Signal. Behav. 6:583-585(2011).
Subcellular Location
Endosome membrane.
UNIPROT
Synonyms
1-phosphatidylinositol-3-phosphate 5-kinase FAB1A
Phosphatidylinositol 3-phosphate 5-kinase
FYVE finger-containing phosphoinositide kinase
PIKfyve
Phosphatidylinositol 3-phosphate 5-kinase type III
PIPkin-III
Type III PIP kinase
Protein FORMS APLOID AND BINUCLEATE CELLS 1A
FAB1A
At4g33240
ExpandImages
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Immunofluorescence staining of paraffin- embedded A. thaliana using anti-FAB1A rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with HA500403 at 1/100 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-FAB1A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500403, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"