PSMB2 Rabbit Polyclonal Antibody
Catalog# HA500410
PSMB2 Rabbit Polyclonal Antibody
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WB
-
IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PSMB2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within C-terminal human PSMB2.
Species Reactivity
Human (Predicted: Mouse, Rat)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 23 kDa
Positive Control
Jurkat cell lysate, 293T cell lysate, Hela cell lysate, CAL62 cell lysate, human colon tissue, human kidney tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1.9 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:10,000
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IHC-P
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1:2,000-1:5,000
Target
Function
Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7.
Background References
1. Rut W., Drag M. Human 20S proteasome activity towards fluorogenic peptides of various chain lengths. Biol. Chem. 397:921-926(2016).
2. Yano M., Koumoto Y., Kanesaki Y., Wu X., Kido H. 20S proteasome prevents aggregation of heat-denatured proteins without PA700 regulatory subcomplex like a molecular chaperone. Biomacromolecules 5:1465-1469(2004)
Subcellular Location
Cytoplasm, Nucleus.
UNIPROT
Synonyms
HC7 I antibody
Macropain subunit C7 I antibody
Macropain subunit C7-I antibody
Multicatalytic endopeptidase complex subunit C7 1 antibody
Multicatalytic endopeptidase complex subunit C7 I antibody
Multicatalytic endopeptidase complex subunit C7-I antibody
Proteasome (prosome, macropain) subunit beta type 2 antibody
Proteasome beta 2 subunit antibody
Proteasome component C7 I antibody
Proteasome component C7-I antibody
ExpandImages
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Western blot analysis of PSMB2 on different lysates with Rabbit anti-PSMB2 antibody (HA500410) at 1/5,000 dilution.
Lane 1: Jurkat
Lane 2: HeLa
Lane 3: Hep G2
Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500410, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 22.8 kDa
Observed band size: 23 kDa -
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PSMB2 antibody (HA500410) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500410) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PSMB2 antibody (HA500410) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500410) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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