WNT9B Rabbit Polyclonal Antibody
Catalog# HA500412
WNT9B Rabbit Polyclonal Antibody
-
WB
-
Human
-
Mouse
-
unconjugated
Overview
Product Name
WNT9B Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human WNT9B aa 100-357.
Species Reactivity
Human, Mouse
Validated Applications
WB
Molecular Weight
Predicted band size: 39 kDa
Positive Control
Mouse kidney tissue lysate, human kidney tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:1,000-1:5,000
Target
Function
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal embryonic kidney development, and for normal development of the urogenital tract, including uterus and part of the oviduct and the upper vagina in females, and epididymis and vas deferens in males. Activates a signaling cascade in the metanephric mesenchyme that induces tubulogenesis. Acts upstream of WNT4 in the signaling pathways that mediate development of kidney tubules and the Muellerian ducts. Plays a role in cranofacial development and is required for normal fusion of the palate during embryonic development. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region. Alternative splicing results in multiple transcript variants. Moderately expressed in fetal kidney and adult kidney. Also found in brain.
Background References
1. Bourhis E., Tam C., Franke Y., Bazan J.F., Ernst J., Hwang J., Costa M., Cochran A.G., Hannoush R.N. Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6. J. Biol. Chem. 285:9172-9179(2010)
Subcellular Location
Extracellular region or secreted.
UNIPROT
Synonyms
MGC124412 antibody
Protein Wnt 9b precursor antibody
Protein Wnt-14b antibody
Protein Wnt-9b antibody
Wingless type MMTV integration site 9B antibody
Wingless type MMTV integration site family member 15 antibody
Wingless type MMTV integration site family member 9B antibody
Wnt 14b antibody
Wnt-15 antibody
Wnt14b antibody
ExpandImages
-
Western blot analysis of WNT9B on different lysates with Rabbit anti-WNT9B antibody (HA500412) at 1/1,000 dilution.
Lane 1: Human kidney tissue lysate
Lane 2: Mouse kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 42 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500412) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"