LRRTM1 Rabbit Polyclonal Antibody
Catalog# HA500418
LRRTM1 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
LRRTM1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within mouse LRRTM1 aa 320-360 / 522 (Extracellular).
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 59 kDa
Positive Control
SH-SY5Y cell lysate, N2A cell lysate, rat brain tissue lysate, N2A, SH-SY5Y.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 1% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:50-1:100
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FC
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1:100-1:500
Target
Function
LRRTM1 Antibody (A-2) is a high quality monoclonal LRRTM1 antibody (also designated LRRTM1 antibody) suitable for the detection of the LRRTM1 protein of mouse, rat and human origin. LRRTM1 Antibody (A-2) is available as both the non-conjugated anti-LRRTM1 antibody form, as well as multiple conjugated forms of anti-LRRTM1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The leucine-rich repeat (LRR) is a 20-30 amino acid motif that forms a hydrophobic α/β horseshoe fold, allowing it to accommodate several leucine residues within a tightly packed core. All LRRs contain a variable segment and a highly conserved segment, the latter of which accounts for 11 or 12 residues of the entire LRR motif. LRRTM1 (leucine rich repeat transmembrane neuronal 1) is a 522 amino acid single-pass type I membrane protein that localizes to the endoplasmic reticulum and contains ten LRRs. Expressed predominately in forebrain tissue, LRRTM1 is thought to be involved in the development of forebrain structures, specifically by influencing axon trafficking, as well as neuronal differentiation and connectivity. Human LRRTM1 shares 96% amino acid identity with its mouse counterpart, suggesting a conserved role between species. Defects in the gene encoding LRRTM1 may be associated with the pathogenesis of several common neurodevelopmental disorders.
Background References
1. Bhouri M. et. al. Deletion of LRRTM1 and LRRTM2 in adult mice impairs basal AMPA receptor transmission and LTP in hippocampal CA1 pyramidal neurons. Proc Natl Acad Sci U S A. 2018 Jun
2. Monavarfeshani A. et. al. LRRTM1 underlies synaptic convergence in visual thalamus. Elife. 2018 Feb
Subcellular Location
Cell membrane, postsynaptic cell membrane.
Synonyms
4632401D06Rik antibody
AW125451 antibody
FLJ32082 antibody
Leucine rich repeat transmembrane neuronal 1 antibody
Leucine rich repeat transmembrane neuronal 1 protein antibody
Leucine-rich repeat transmembrane neuronal protein 1 antibody
LRRT1_HUMAN antibody
LRRTM1 antibody
Lrrtm1 leucine rich repeat transmembrane neuronal 1 antibody
OTTHUMP00000194829 antibody
Images
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Western blot analysis of LRRTM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500418, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SH-SY5Y cell lysate
Lane 2: N2A cell lysate
Lane 1: Rat brain tissue lysate -
ICC staining of LRRTM1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500418, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Flow cytometric analysis of LRRTM1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500418, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"