Naked1 Rabbit Polyclonal Antibody
Catalog# HA500419
Naked1 Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Naked1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human Naked1 aa 30-100.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Cell
Target Molecular Weight
Predicted band size: 52 kDa
Positive Control
HepG2 cell lysate, HCT116 cell lysate, 293 cell lysate, SH-SY5Y cell lysate, rat smooth muscle tissue, mouse smooth muscle tissue, SH-SY5Y.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
2.107 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IHC-P
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1:400
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IF-Cell
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1:200
Target
Function
Naked cuticle 1 (NKD1) is a human gene that encodes the protein Nkd1, a member of the Naked cuticle (Nkd) family of proteins that regulate the Wnt signaling pathway. Insects typically have a single Nkd gene, whereas there are two Nkd genes, Nkd1 and Nkd2, in most vertebrates studied to date (zebrafish appear to have additional homologous genes such as Nkd3). Nkd1 binds to the Dishevelled (Dvl) family of proteins (DVL1, DVL2, DVL3). Specific truncating NKD1 mutations identified in DNA mismatch repair deficient colon cancer that disrupt Nkd1/Dvl binding implicate these mutations as a cause of increased Wnt signaling in approximately 1% of human colon cancer, the majority of which have increased Wnt signaling due to mutations the adenomatous polyposis coli (APC), AXIN2, or rarely the beta-catenin genes.
Background References
1. Van Raay TJ, Coffey RJ, Solnica-Krezel L. Zebrafish Naked1 and Naked2 antagonize both canonical and non-canonical Wnt signaling. Dev Biol. 2007 Sep 15;309(2):151-68. Epub 2007 Apr 21.
2. Schneider I, Schneider PN, Derry SW, Lin S, Barton LJ, Westfall T, Slusarski DC. Zebrafish Nkd1 promotes Dvl degradation and is required for left-right patterning. Dev Biol. 2010 Dec 1;348(1):22-33. Epub 2010 Sep 19.
3. Guo J, Cagatay T, Zhou G, Chan CC, Blythe S, Suyama K, Zheng L, Pan K, Qian C, Hamelin R, Thibodeau SN, Klein PS, Wharton KA, Liu W. Mutations in the human naked cuticle homolog NKD1 found in colorectal cancer alter Wnt/Dvl/beta-catenin signaling. PLoS One. 2009 Nov 24;4(11):e7982.
Subcellular Location
Cell membrane, Cytoplasm, Membrane.
Synonyms
Dvl-binding protein antibody
Naked cuticle 1 antibody
naked cuticle 1 homolog antibody
Naked cuticle homolog 1 (Drosophila) antibody
Naked-1 antibody
NKD antibody
Nkd1 antibody
Protein naked cuticle homolog 1 antibody
Images
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Western blot analysis of Naked1 on different lysates with Rabbit anti-Naked1 antibody (HA500419) at 1/500 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HCT116 cell lysate
Lane 3: 293 cell lysate
Lane 4: SH-SY5Y cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500419) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue with Rabbit anti-Naked1 antibody (HA500419) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500419) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue with Rabbit anti-Naked1 antibody (HA500419) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500419) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunocytochemistry analysis of SH-SY5Y cells labeling Naked1 with Rabbit anti-Naked1 antibody (HA500419) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Naked1 antibody (HA500419) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"