IFNAR2 Rabbit Polyclonal Antibody
Catalog# HA500425
IFNAR2 Rabbit Polyclonal Antibody
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WB
-
Human
-
Rat
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unconjugated
Overview
Product Name
IFNAR2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human IFNAR2 aa 466-515 / 515.
Species Reactivity
Human, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 58 kDa
Positive Control
Hela cell lysate, SW480 cell lysate, LO2 cell lysate, rat liver tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500
Target
Function
Interferon-alpha/beta receptor beta chain is a protein that in humans is encoded by the IFNAR2 gene. The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Multiple transcript variants encoding at least two different isoforms have been found for this gene.
Background References
1. Qing J et al. Identification of Interferon Receptor IFNAR2 As a Novel HCV Entry Factor by Using Chemical Probes. ACS Chem Biol. 2020 May
2. Shemesh M et al. IFNAR1 and IFNAR2 play distinct roles in initiating type I interferon-induced JAK-STAT signaling and activating STATs. Sci Signal. 2021 Nov
Subcellular Location
Cell membrane; Secreted.
Synonyms
Human interferon alpha/beta receptor antibody
IFN alpha REC antibody
IFN R antibody
IFN-a/ßR2 antibody
IFN-a/ßRß antibody
IFN-alpha binding protein antibody
IFN-alpha/beta receptor 2 antibody
IFN-R-2 antibody
IFNABR antibody
IFNAR2 antibody
ExpandImages
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Western blot analysis of IFNAR2 on different lysates with Rabbit anti-IFNAR2 antibody (HA500425) at 1/500 dilution.
Lane 1: Hela cell lysate (30 µg/Lane)
Lane 2: SW480 cell lysate (10 µg/Lane)
Lane 3: LO2 cell lysate (20 µg/Lane)
Lane 4: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 58 kDa
Observed band size: 58/95/100 kDa (Predicted glycosylation modification)
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500425) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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