TRRAP Rabbit Polyclonal Antibody
Catalog# HA500431
TRRAP Rabbit Polyclonal Antibody
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WB
-
Human
-
Rat
-
unconjugated
Overview
Product Name
TRRAP Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human TRRAP aa 2,601-2,910 / 3,859.
Species Reactivity
Human, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 438 kDa
Positive Control
K-562 cell lysate, 293T cell lysate, PC-12 cell lysate, L6 cell lysate, PMVEC cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Adapter protein, which is found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), which gives a specific tag for epigenetic transcription activation. Component of the NuA4 histone acetyltransferase complex which is responsible for acetylation of nucleosomal histones H4 and H2A. Plays a central role in MYC transcription activation, and also participates in cell transformation by MYC. Required for p53/TP53-, E2F1- and E2F4-mediated transcription activation. Also involved in transcription activation mediated by the adenovirus E1A, a viral oncoprotein that deregulates transcription of key genes. Probably acts by linking transcription factors such as E1A, MYC or E2F1 to HAT complexes such as STAGA thereby allowing transcription activation. Probably not required in the steps following histone acetylation in processes of transcription activation. May be required for the mitotic checkpoint and normal cell cycle progression. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome. May play a role in the formation and maintenance of the auditory system (By similarity).
Background References
1. Lang SE, McMahon SB, Cole MD, Hearing P. E2F transcriptional activation requires TRRAP and GCN5 cofactors. J Biol Chem. 2001 Aug 31;276(35):32627-34.
2. Ard PG, Chatterjee C, Kunjibettu S, Adside LR, Gralinski LE, McMahon SB. Transcriptional regulation of the mdm2 oncogene by p53 requires TRRAP acetyltransferase complexes. Mol Cell Biol. 2002 Aug;22(16):5650-61.
Subcellular Location
Nucleus.
Synonyms
350/400 kDa PCAF associated factor antibody
PAF 350/400 antibody
PAF 400 antibody
PAF350/400 antibody
PAF400 antibody
STAF 40 antibody
STAF40 antibody
TR AP antibody
Tra 1 antibody
Tra1 antibody
ExpandImages
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Western blot analysis of TRRAP on different lysates with Rabbit anti-TRRAP antibody (HA500431) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: 293T cell lysate
Lysates/proteins at 25 µg/Lane.
Predicted band size: 438 kDa
Observed band size: 438 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500431) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of TRRAP on different lysates with Rabbit anti-TRRAP antibody (HA500431) at 1/1,000 dilution.
Lane 1: PC-12 cell lysate
Lane 2: L6 cell lysate
Lane 3: PMVEC cell lysate
Predicted band size: 438 kDa
Observed band size: 438 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500431) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"