TMK4 Rabbit Polyclonal Antibody
Catalog# HA500435
TMK4 Rabbit Polyclonal Antibody
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IF-Tissue
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IHC-P
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Arabidopsis thaliana
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unconjugated
Overview
Product Name
TMK4 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within A. thaliana TMK4 aa 700-928 / 928.
Species Reactivity
Arabidopsis thaliana
Validated Applications
IF-Tissue, IHC-P
Target Molecular Weight
100 kDa
Positive Control
A. thaliana tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 1% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:400
Target
Function
Involved in auxin signal transduction and cell expansion and proliferation regulation. May be involved in brassinosteroid-mediated plant growth and development via auxin regulation. May be involved in microspore and pollen development.
Background References
1. Liang X. et. al. Receptor-Like Cytoplasmic Kinases: Central Players in Plant Receptor Kinase-Mediated Signaling. Annu Rev Plant Biol. (2018).
Subcellular Location
Membrane
UNIPROT
Synonyms
Receptor-like kinase TMK4
BAK1-associating receptor-like kinase 11 Publication
Leucine-rich repeat receptor-like kinases TMK41 Publication
Transmembrane kinase 4
TMK4
BARK1
At3g23750
MYM9.9
Images
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Immunofluorescence staining of paraffin- embedded A. thaliana using anti-TMK4 rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with HA500435 at 1/100 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-TMK4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500435, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"