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RBR-type E3 ubiquitin transferase Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500436

RBR-type E3 ubiquitin transferase Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Rice

Conjugation

  • unconjugated

Overview

Product Name

RBR-type E3 ubiquitin transferase Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide corresponding to Rice RBR-type E3 ubiquitin transferase aa 31-70 / 543.

Species Reactivity

Rice

Validated Applications

WB, IF-Tissue, IHC-P

Molecular Weight

62 kDa

Positive Control

Rice tissue lysates.

Conjugation

unconjugated

RRID

AB_3071521

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

Target

Function

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.

Background References

暂无

UNIPROT

SwissProt: A0A0E0D3V9 Rice

Synonyms

RBR-type E3 ubiquitin transferas antibody

Images

  • Western blot analysis of RBR-type E3 ubiquitin transferase on rice tissue lysate . Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST. in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA500436" style="font-weight: bold;text-decoration: underline;">HA500436</a>, 1/500) was used in 5% NFDM/TBST. at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RBR-type E3 ubiquitin transferase on rice tissue lysate . Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST. in PBS for 1 hour at room temperature. The primary antibody (HA500436, 1/500) was used in 5% NFDM/TBST. at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Immunofluorescence staining of paraffin- embedded rice tissue using anti-RBR-type E3 ubiquitin transferase rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody () at 1/100 dilution for 10 hours at 4℃ and detected using Alexa Fluor&trade; 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

    Immunofluorescence staining of paraffin- embedded rice tissue using anti-RBR-type E3 ubiquitin transferase rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody () at 1/100 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rice tissue using anti-RBR-type E3 ubiquitin transferase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the antibody () at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rice tissue using anti-RBR-type E3 ubiquitin transferase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody () at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"