AP-4 complex subunit epsilon Rabbit Polyclonal Antibody
Catalog# HA500446
AP-4 complex subunit epsilon Rabbit Polyclonal Antibody
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IF-Tissue
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IHC-P
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ELISA
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Arabidopsis thaliana
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unconjugated
Overview
Product Name
AP-4 complex subunit epsilon Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within ARATH AP-4 complex subunit epsilon aa 880-938 / 938.
Species Reactivity
Arabidopsis thaliana
Validated Applications
IF-Tissue, IHC-P, ELISA
Target Molecular Weight
104 kDa
Positive Control
A. thaliana tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IF-Tissue
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1:50-1:100
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IHC-P
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1:50-1:200
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ELISA
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1:10,000
Target
Function
Subunit of novel type of clathrin- or non-clathrin-associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system.
Background References
1. Ivankovic D. et. al. Axonal autophagosome maturation defect through failure of ATG9A sorting underpins pathology in AP-4 deficiency syndrome. Autophagy. 2019 May 29:1-17.
2. De Pace R. et. al. Altered distribution of ATG9A and accumulation of axonal aggregates in neurons from a mouse model of AP-4 deficiency syndrome.PLoS Genet. 2018 Apr 26;14(4):e1007363.
Subcellular Location
Trans-Golgi network, coated pit.
UNIPROT
Synonyms
AP-4 complex subunit epsilon
AP-4 adaptor complex subunit epsilon
Adaptor-related protein complex 4 subunit epsilon
Epsilon subunit of AP-4
Epsilon-adaptin
At1g31730
F27M3.7
Images
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Immunofluorescence staining of paraffin- embedded A. thaliana using anti-AP-4 complex subunit epsilon rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with AP-4 complex subunit epsilon antibody at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-AP-4 complex subunit epsilon antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500446, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"