Skip to content

JAZ11 Rabbit Polyclonal Antibody

Usd: 315

Specification

Bulk Order Quote | Customization Request

Catalog# HA500450

JAZ11 Rabbit Polyclonal Antibody

Application

  • ELISA

  • IF-Tissue

  • IHC-P

Reactivity

  • Rice

Conjugation

  • unconjugated

Overview

Product Name

JAZ11 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within rice JAZ11 aa 160-209 / 209.

Species Reactivity

Rice

Validated Applications

ELISA, IF-Tissue, IHC-P

Molecular Weight

22 kDa

Positive Control

Rice tissue.

Conjugation

unconjugated

RRID

AB_3071535

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • ELISA

  • 1:10,000

  • IF-Tissue

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

Target

Function

Repressor of jasmonate responses.

Background References

1. Song S. et. al. The Jasmonate-ZIM domain proteins interact with the R2R3-MYB transcription factors MYB21 and MYB24 to affect Jasmonate-regulated stamen development in Arabidopsis. Plant Cell. 2011 Mar;23(3):1000-13.

Subcellular Location

Nucleus.

UNIPROT

SwissProt:Q8GRS2 ORYSJ

Synonyms

Protein TIFY 11c

OsTIFY11c

Jasmonate ZIM domain-containing protein 11

OsJAZ11

OsJAZ2

TIFY11C

JAZ11

Os03g0180900

LOC_Os03g08320

OsJ_09657

Expand

Images

  • Immunofluorescence staining of paraffin- embedded rice tissue using anti-JAZ11 rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with JAZ11 antibody at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

    Immunofluorescence staining of paraffin- embedded rice tissue using anti-JAZ11 rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with JAZ11 antibody at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded rice tissue using anti-JAZ11 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500450" style="font-weight: bold;text-decoration: underline;">HA500450</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rice tissue using anti-JAZ11 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500450, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"