WDR77 Rabbit Polyclonal Antibody
Catalog# HA500456
WDR77 Rabbit Polyclonal Antibody
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WB
-
IF-Cell
-
FC
-
Human
-
unconjugated
Overview
Product Name
WDR77 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human WDR77 aa 1-342 / 342.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 37 kDa
Positive Control
HepG2 cell lysate, Daudi cell lysate, Hela cell lysate, 293 cell lysate, K562 cell lysate, Hela, HepG2.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:1,000
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IF-Cell
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1:200
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FC
-
1:500-1:1,000
Target
Function
Methylosome protein 50 is a protein that in humans is encoded by the WDR77 gene. Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
Background References
1. Yuan H et al. HBx represses WDR77 to enhance HBV replication by DDB1-mediated WDR77 degradation in the liver. Theranostics. 2021 Jul
2. Zhao Y et al. Germ-line mutations in WDR77 predispose to familial papillary thyroid cancer. Proc Natl Acad Sci U S A. 2021 Aug
Subcellular Location
Nucleus, Cytoplasm.
UNIPROT
Synonyms
2610312E17Rik antibody
Androgen receptor cofactor p44 antibody
C79984 antibody
HKMT1069 antibody
MEP 50 antibody
MEP-50 antibody
MEP50 antibody
MEP50_HUMAN antibody
Methylosome protein 50 antibody
MGC2722 antibody
ExpandImages
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Western blot analysis of WDR77 on different lysates with Rabbit anti-WDR77 antibody (HA500456) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: Daudi cell lysate
Lane 3: Hela cell lysate
Lane 4: 293 cell lysate
Lane 5: K562 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 40 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500456) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Hela cells labeling WDR77 with Rabbit anti-WDR77 antibody (HA500456) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-WDR77 antibody (HA500456) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Flow cytometric analysis of HepG2 cells labeling WDR77.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500456, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of Hela cells labeling WDR77.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500456, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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