PSMB3 Rabbit Polyclonal Antibody
Catalog# HA500458
PSMB3 Rabbit Polyclonal Antibody
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WB
-
IF-Cell
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IHC-P
-
FC
-
Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PSMB3 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant full length protein within human PSMB3.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC
Molecular Weight
Predicted band size: 23 kDa
Positive Control
Hela cell lysate, K562 cell lysate, 293T cell lysate, Jurkat cell lysate, HeLa, Human skin tissue, Human breast tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
-
1:1,000
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IF-Cell
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1:200
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IHC-P
-
1:500-1:1,000
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FC
-
1:1,000
Target
Function
Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
Background References
1. Yano M., Koumoto Y., Kanesaki Y., Wu X., Kido H. 20S proteasome prevents aggregation of heat-denatured proteins without PA700 regulatory subcomplex like a molecular chaperone. Biomacromolecules 5:1465-1469 (2004)
2. Rut W., Drag M. Human 20S proteasome activity towards fluorogenic peptides of various chain lengths. Biol. Chem. 397:921-926 (2016)
Subcellular Location
Cytoplasm, Nucleus, Proteasome
Synonyms
C10 II antibody
HC10 II antibody
proteasome (prosome, macropain) subunit, beta type 3 antibody
Proteasome beta 3 subunit antibody
Proteasome chain 13 antibody
proteasome component C10 II antibody
Proteasome component C10-II antibody
Proteasome subunit beta type-3 antibody
Proteasome theta chain antibody
PSB3_HUMAN antibody
ExpandImages
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Western blot analysis of PSMB3 on different lysates with Rabbit anti-PSMB3 antibody (HA500458) at 1/1,000 dilution.
Lane 1: Hela cell lysate (10 µg/Lane)
Lane 2: K562 cell lysate (10 µg/Lane)
Lane 3: 293T cell lysate (10 µg/Lane)
Lane 4: Jurkat cell lysate (10 µg/Lane)
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 2 minutes;
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500458) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/300,000 dilution was used for 1 hour at room temperature. Rabbit anti-GAPDH antibody (ET1601-4) was used as loading control antibody at 1:10,000 dilution. -
Immunocytochemistry analysis of HeLa cells labeling PSMB3 with Rabbit anti-PSMB3 antibody (HA500458) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.05% Triton X-100 in PBS for 15 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMB3 antibody (HA500458) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Mouse anti-Beta tubulin Antibody (M1305-2) was used as Microtubule Marker at 1/200 dilution and Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded Human skin tissue with Rabbit anti-PSMB3 antibody (HA500458) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500458) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded Human breast tissue with Rabbit anti-PSMB3 antibody (HA500458) at 1/600 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500458) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-PSMB3 antibody (HA500458) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500458) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-PSMB3 antibody (HA500458) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500458) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of HeLa cells labeling PSMB3.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500458, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"