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BCL9L Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500459

BCL9L Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

BCL9L Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within Human BCL9L aa 351-550 / 1,499.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 157 kDa

Positive Control

Hela cell lysate, MCF-7 cell lysate, A549 cell lysate, SW480 cell lysate, mouse skin tissue, rat skin tissue, human cervical cancer tissue, human lung carcinoma tissue, human liver tissue.

Conjugation

unconjugated

RRID

AB_3071544

Product Features

Form

Liquid

Concentration

1.861 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:1,000

Target

Function

Transcriptional regulator that acts as an activator. Promotes beta-catenin transcriptional activity. Plays a role in tumorigenesis. Enhances the neoplastic transforming activity of CTNNB1. In mammalian cells, BCL9 proteins contribute to, but may not be obligatory for, β-catenin–mediated transcription. BCL9L mRNA knockdown in a human colon cancer cell line induced an epithelial phenotype that was associated with the translocation of β-catenin from the nucleus to the cell membrane, suggesting that BCL9L might regulate the switch between the adhesive and transcriptional functions of β-catenin. BCL9L upregulation has been associated with breast and colon cancers, and BCL9 has recently been reported to enhance β-catenin–mediated transcription and increase the proliferation as well as the metastatic potential of tumor cells.

Background References

1. Wang X et al. BCL9/BCL9L promotes tumorigenicity through immune-dependent and independent mechanisms in triple negative breast cancer. Oncogene. 2021 Apr

2. Vafaizadeh V et al. The interactions of Bcl9/Bcl9L with β-catenin and Pygopus promote breast cancer growth, invasion, and metastasis. Oncogene. 2021 Oct

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q86UU0 Human

SwissProt: Q67FY2 Mouse

Entrez Gene: 300673 Rat

Synonyms

B-cell CLL antibody

B-cell CLL/lymphoma 9-like protein antibody

B-cell lymphoma 9-like protein antibody

B9L antibody

BCL9-2 antibody

BCL9-like protein antibody

BCL9-related beta-catenin-binding protein antibody

Bcl9l antibody

BCL9L_HUMAN antibody

DLNB11 antibody

Expand

Images

  • Western blot analysis of BCL9L on different lysates with Rabbit anti-BCL9L antibody (<a href="/products/HA500459" style="font-weight: bold;text-decoration: underline;">HA500459</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: MCF7 cell lysate<br />Lane 3: A549 cell lysate<br />Lane 4: SW480 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 35 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500459" style="font-weight: bold;text-decoration: underline;">HA500459</a>, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 157 kDa<br />Observed band size: 190 kDa

    Western blot analysis of BCL9L on different lysates with Rabbit anti-BCL9L antibody (HA500459) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: MCF7 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: SW480 cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 35 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500459, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 157 kDa
    Observed band size: 190 kDa

  • Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-BCL9L antibody (<a href="/products/HA500459" style="font-weight: bold;text-decoration: underline;">HA500459</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500459" style="font-weight: bold;text-decoration: underline;">HA500459</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue with Rabbit anti-BCL9L antibody (HA500459) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500459) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-BCL9L antibody (<a href="/products/HA500459" style="font-weight: bold;text-decoration: underline;">HA500459</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500459" style="font-weight: bold;text-decoration: underline;">HA500459</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-BCL9L antibody (HA500459) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500459) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue with Rabbit anti-BCL9L antibody (<a href="/products/HA500459" style="font-weight: bold;text-decoration: underline;">HA500459</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500459" style="font-weight: bold;text-decoration: underline;">HA500459</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue with Rabbit anti-BCL9L antibody (HA500459) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500459) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-BCL9L antibody (<a href="/products/HA500459" style="font-weight: bold;text-decoration: underline;">HA500459</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500459" style="font-weight: bold;text-decoration: underline;">HA500459</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-BCL9L antibody (HA500459) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500459) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-BCL9L antibody (<a href="/products/HA500459" style="font-weight: bold;text-decoration: underline;">HA500459</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500459" style="font-weight: bold;text-decoration: underline;">HA500459</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-BCL9L antibody (HA500459) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500459) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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