AP2A2 Rabbit Polyclonal Antibody
Catalog# HA500477
AP2A2 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
AP2A2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human AP2A2 aa 740-939 / 939.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
104 kDa
Positive Control
Hela cell lysate, mouse cerebellum tissue lysate, rat brain tissue lysate, human kidney tissue, mouse cerebellum tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:5,000
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IHC-P
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1:200-1:1,000
Target
Function
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10.
Background References
1. Nelson PT. et. al. The MUC6/AP2A2 Locus and Its Relevance to Alzheimer\'s Disease: A Review. J Neuropathol Exp Neurol. 2020 Jun
2. Montgomery MK. et. al. The role of Ap2a2 in PPARalpha-mediated regulation of lipolysis in adipose tissue. FASEB J. 2019 Dec
Subcellular Location
Cell membrane, coated pit.
Synonyms
100 kDa coated vesicle protein C antibody
Adaptor protein complex AP-2 subunit alpha-2 antibody
Adaptor-related protein complex 2 subunit alpha-2 antibody
Alpha-adaptin C antibody
Alpha2-adaptin antibody
AP-2 complex subunit alpha-2 antibody
Ap2a2 antibody
AP2A2_HUMAN antibody
Clathrin assembly protein complex 2 alpha-C large chain antibody
HIP-9 antibody
ExpandImages
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Western blot analysis of AP2A2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500477, 1/2,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: Mouse cerebellum tissue lysate
Lane 3: Rat brain tissue lysate -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AP2A2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500477, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-AP2A2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500477, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"