NARS Rabbit Polyclonal Antibody
Catalog# HA500479
NARS Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
NARS Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human NARS aa 281-480 / 548.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 63 kDa
Positive Control
HepG2 cell lysate, K562 cell lysate, 293 cell lysate, NIH/3T3 cell lysate, Mouse liver tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
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1:1,000-1:5,000
Target
Function
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases.
Background References
1. Wang L. et. al. Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly. Nat Commun. 2020 Aug
2. Manole A. et. al. De Novo and Bi-allelic Pathogenic Variants in NARS1 Cause Neurodevelopmental Delay Due to Toxic Gain-of-Function and Partial Loss-of-Function Effects. Am J Hum Genet. 2020 Aug
Subcellular Location
Cytoplasm.
Synonyms
3010001M15Rik antibody
AA960128 antibody
AsnRS antibody
ASNS antibody
Asparagine tRNA ligase 1, cytoplasmic antibody
Asparagine tRNA ligase antibody
Asparagine--tRNA ligase antibody
Asparaginyl tRNA synthetase antibody
Asparaginyl-tRNA synthetase antibody
C78150 antibody
ExpandImages
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Western blot analysis of NARS on different lysates with Rabbit anti-NARS antibody (HA500479) at 1/5,000 dilution.
Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate（20μg /Lane）
Lane 2: HepG2 (Human hepatocellular carcinoma cell) cell lysate（20μg /Lane）
Lane 3: Mouse liver tissue lysate（40μg /Lane）
Lane 4: Rat brain tissue lysate（40μg /Lane）
Lane 5: Rat liver tissue lysate（40μg /Lane）
Exposure time: 46 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500479, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 63 kDa
Observed band size: 63 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"