VGLUT1 Rabbit Polyclonal Antibody
Catalog# HA500488
VGLUT1 Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
VGLUT1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human VGluT1 aa 1-63 / 560.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
62 kDa
Positive Control
K562 cell lysates, mouse brain tissue lysate, rat brain tissue lysate, mouse cerebellum tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
Target
Function
Vesicular glutamate transporter 1 (VGLUT1) is a protein that in humans is encoded by the SLC17A7 gene. The protein encoded by this gene is a vesicle-bound, sodium-dependent phosphate transporter that is specifically expressed in the neuron-rich regions of the brain. It is preferentially associated with the membranes of synaptic vesicles and functions in glutamate transport. The protein shares 82% identity with the differentiation-associated Na-dependent inorganic phosphate cotransporter and they appear to form a distinct class within the Na+/Pi cotransporter family.
Background References
1. Li X. et. al. SLC17A7 gene may be the indicator of selective serotonin reuptake inhibitor treatment response in the Chinese Han population. J Clin Psychopharmacol. 2014 Jun
2. Madura SA. et. al. Genetic variation in SLC17A7 promoter associated with response to sport-related concussions. Brain Inj. 2016
Subcellular Location
Synaptic vesicle membrane, synaptosome.
Synonyms
BNPI antibody
Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
Brain specific Na dependent inorganic phosphate cotransporter antibody
Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody
Slc17a7 antibody
Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody
Solute carrier family 17 member 7 antibody
Vesicular glutamate transporter 1 antibody
VGLU1_HUMAN antibody
VGLUT 1 antibody
ExpandImages
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Western blot analysis of VGLUT1 on different lysates with Rabbit anti-VGLUT1 antibody (HA500488) at 1/1,000 dilution.
Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate (15 µg/Lane)
Lane 2: 293T (Human embryonic kidney cell) cell lysate (15 µg/Lane)
Lane 3: Mouse brain tissue lysate (30 µg/Lane)
Lane 4: Rat brain tissue lysate(30 µg/Lane)
Exposure time: 120 seconds ; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500488, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 62 kDa
Observed band size: 62 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
VGLUT1 Mouse Monoclonal Antibody [A7A3]
Application: WB
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
