Cytokeratin 5+6 Rabbit Polyclonal Antibody
Catalog# HA500508
Cytokeratin 5+6 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
Cytokeratin 5+6 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human Cytokeratin 5 aa 191-240.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
62/60 kDa
Positive Control
Human skin tissue lysate, A431 cell lysate, human tonsil tissue, human lung adenocarcinoma tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:600
Target
Function
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin (KRT5) is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
Background References
1. Akhtar M. et. al. CK20 and CK5/6 Immunohistochemical Staining of Urothelial Neoplasms: A Perspective. Adv Urol. 2020 Nov
2. Batool S. et. al. Role Of Alteration Of Ck5\6 Profile In Dysplastic Progression Of Oral Mucosa In Tobacco Users. J Ayub Med Coll Abbottabad. 2020 Oct-Dec
Subcellular Location
Intermediate filament, Keratin.
UNIPROT
Synonyms
58 kDa cytokeratin antibody
CK-5 antibody
CK5 antibody
Cytokeratin-5 antibody
Cytokeratin5 antibody
DDD antibody
DDD1 antibody
EBS2 antibody
epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody
K2C5_HUMAN antibody
ExpandImages
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Western blot analysis of Cytokeratin 5+6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500508, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human skin tissue lysate
Lane 2: A431 cell lysate -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 5+6 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500508, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using anti-Cytokeratin 5+6 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500508, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"